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Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains

Investigating the genetic diversity and population structure is important in conserving narrowly distributed plants. In this study, 90 Clematis acerifolia (C. acerifolia) plants belonging to nine populations were collected from the Taihang Mountains in Beijing, Hebei, and Henan. Twenty-nine simple s...

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Autores principales: Zhao, Zhengnan, Zhang, Hongwei, Wang, Pingxi, Yang, Yuan, Sun, Hongyan, Li, Jinyu, Chen, Xiao, Li, Jun, Ji, Naizhe, Feng, Hui, Zhao, Shiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10198494/
https://www.ncbi.nlm.nih.gov/pubmed/37205665
http://dx.doi.org/10.1371/journal.pone.0285754
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author Zhao, Zhengnan
Zhang, Hongwei
Wang, Pingxi
Yang, Yuan
Sun, Hongyan
Li, Jinyu
Chen, Xiao
Li, Jun
Ji, Naizhe
Feng, Hui
Zhao, Shiwei
author_facet Zhao, Zhengnan
Zhang, Hongwei
Wang, Pingxi
Yang, Yuan
Sun, Hongyan
Li, Jinyu
Chen, Xiao
Li, Jun
Ji, Naizhe
Feng, Hui
Zhao, Shiwei
author_sort Zhao, Zhengnan
collection PubMed
description Investigating the genetic diversity and population structure is important in conserving narrowly distributed plants. In this study, 90 Clematis acerifolia (C. acerifolia) plants belonging to nine populations were collected from the Taihang Mountains in Beijing, Hebei, and Henan. Twenty-nine simple sequence repeats (SSR) markers developed based on RAD-seq data were used to analyze the genetic diversity and population structure of C. acerifolia. The mean PIC value for all markers was 0.2910, indicating all SSR markers showed a moderate degree of polymorphism. The expected heterozygosity of the whole populations was 0.3483, indicating the genetic diversity of both C. acerifolia var. elobata and C. acerifolia were low. The expected heterozygosity of C. acerifolia var. elobata (He = 0.2800) was higher than that of C. acerifolia (He = 0.2614). Genetic structure analysis and principal coordinate analysis demonstrated that C. acerifolia and C. acerifolia var. elobata showed great genetic differences. Molecular variance analysis (AMOVA) demonstrated that within-population genetic variation (68.31%) was the main contributor to the variation of the C. acerifolia populations. Conclusively, C. acerifolia var. elobata had higher genetic diversity than C. acerifolia, and there are significant genetic differences between C. acerifolia and C. acerifolia var. elobata, and small genetic variations within the C. acerifolia populations. Our results provide a scientific and rational basis for the conservation of C. acerifolia and provide a reference for the conservation of other cliff plants.
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spelling pubmed-101984942023-05-20 Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains Zhao, Zhengnan Zhang, Hongwei Wang, Pingxi Yang, Yuan Sun, Hongyan Li, Jinyu Chen, Xiao Li, Jun Ji, Naizhe Feng, Hui Zhao, Shiwei PLoS One Research Article Investigating the genetic diversity and population structure is important in conserving narrowly distributed plants. In this study, 90 Clematis acerifolia (C. acerifolia) plants belonging to nine populations were collected from the Taihang Mountains in Beijing, Hebei, and Henan. Twenty-nine simple sequence repeats (SSR) markers developed based on RAD-seq data were used to analyze the genetic diversity and population structure of C. acerifolia. The mean PIC value for all markers was 0.2910, indicating all SSR markers showed a moderate degree of polymorphism. The expected heterozygosity of the whole populations was 0.3483, indicating the genetic diversity of both C. acerifolia var. elobata and C. acerifolia were low. The expected heterozygosity of C. acerifolia var. elobata (He = 0.2800) was higher than that of C. acerifolia (He = 0.2614). Genetic structure analysis and principal coordinate analysis demonstrated that C. acerifolia and C. acerifolia var. elobata showed great genetic differences. Molecular variance analysis (AMOVA) demonstrated that within-population genetic variation (68.31%) was the main contributor to the variation of the C. acerifolia populations. Conclusively, C. acerifolia var. elobata had higher genetic diversity than C. acerifolia, and there are significant genetic differences between C. acerifolia and C. acerifolia var. elobata, and small genetic variations within the C. acerifolia populations. Our results provide a scientific and rational basis for the conservation of C. acerifolia and provide a reference for the conservation of other cliff plants. Public Library of Science 2023-05-19 /pmc/articles/PMC10198494/ /pubmed/37205665 http://dx.doi.org/10.1371/journal.pone.0285754 Text en © 2023 Zhao et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhao, Zhengnan
Zhang, Hongwei
Wang, Pingxi
Yang, Yuan
Sun, Hongyan
Li, Jinyu
Chen, Xiao
Li, Jun
Ji, Naizhe
Feng, Hui
Zhao, Shiwei
Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title_full Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title_fullStr Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title_full_unstemmed Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title_short Development of SSR molecular markers and genetic diversity analysis of Clematis acerifolia from Taihang Mountains
title_sort development of ssr molecular markers and genetic diversity analysis of clematis acerifolia from taihang mountains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10198494/
https://www.ncbi.nlm.nih.gov/pubmed/37205665
http://dx.doi.org/10.1371/journal.pone.0285754
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