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Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer. PTEN was validated by dual luciferas...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199038/ https://www.ncbi.nlm.nih.gov/pubmed/37208413 http://dx.doi.org/10.1038/s41598-023-35059-x |
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author | Mohammaddoust, Samaneh Sadeghizadeh, Majid |
author_facet | Mohammaddoust, Samaneh Sadeghizadeh, Majid |
author_sort | Mohammaddoust, Samaneh |
collection | PubMed |
description | Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer. PTEN was validated by dual luciferase assay as a target gene of miR-183. Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. To determine the impacts of miR-183 on cell viability, the MTT assay was used. Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. Western blot was utilized to assess the effect of miR-183 on PTEN protein expression. MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN. According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression. It may be also a potential therapeutic target for this disease. |
format | Online Article Text |
id | pubmed-10199038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101990382023-05-21 Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells Mohammaddoust, Samaneh Sadeghizadeh, Majid Sci Rep Article Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer. PTEN was validated by dual luciferase assay as a target gene of miR-183. Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. To determine the impacts of miR-183 on cell viability, the MTT assay was used. Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. Western blot was utilized to assess the effect of miR-183 on PTEN protein expression. MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN. According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression. It may be also a potential therapeutic target for this disease. Nature Publishing Group UK 2023-05-19 /pmc/articles/PMC10199038/ /pubmed/37208413 http://dx.doi.org/10.1038/s41598-023-35059-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Mohammaddoust, Samaneh Sadeghizadeh, Majid Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title | Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title_full | Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title_fullStr | Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title_full_unstemmed | Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title_short | Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells |
title_sort | mir-183 functions as an oncogene via decreasing pten in breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10199038/ https://www.ncbi.nlm.nih.gov/pubmed/37208413 http://dx.doi.org/10.1038/s41598-023-35059-x |
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