Hand nicotine as an independent marker of thirdhand smoke pollution in children’s environments

BACKGROUND: Hand nicotine (HN) levels measure children’s exposure to tobacco smoke pollutants from thirdhand and secondhand smoke. HN is associated with urinary and salivary cotinine, but the associations of HN with other tobacco smoke exposure (TSE) markers remain unknown. OBJECTIVES: We compared levels of HN and four urinary TSE biomarkers: cotinine, trans-3′-hydroxycotinine (3HC), nicotelline N-oxides, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), and children’s sociodemographic and TSE patterns. We also examined if HN is a plausible pathway for children’s exposure to active smoking. METHODS: Data were collected from 175 non-smoking patients (Mean (SD) age = 5.4 (3.4) years) who lived with ≥1 cigarette smoker(s). HN and TSE biomarker levels were determined using LC-MS/MS. Multivariate and multivariable regression analyses were conducted to examine associations between TSE markers and parent-reported measures, controlling for sociodemographics. RESULTS: Of the five markers of TSE, cotinine ([Formula: see text]) and HN ([Formula: see text]; [Formula: see text]) showed the strongest overall associations. Of the five markers, only cotinine showed significantly higher levels among Black children ([Formula: see text] , [Formula: see text]) independent of age, reported exposure, and home smoking bans. Cotinine ([Formula: see text] , [Formula: see text]), NNAL ([Formula: see text] , [Formula: see text]), and HN ([Formula: see text] , [Formula: see text]) showed significant positive associations with reported exposure independent of race, age, and home smoking bans. NNAL ([Formula: see text] , [Formula: see text]) and HN ([Formula: see text] , [Formula: see text]), but not cotinine, 3HC, and N-oxides, showed significantly lower levels among children who lived in homes with smoking bans. Child age, hand surface area, home smoking ban, and reported exposure independently accounted for 21 % of the variance in HN levels [Formula: see text]. HN accounted for 30 % of the variance in cotinine independent of child race and child age. DISCUSSION: HN levels were associated with modifiable tobacco-related behaviors and shows promise as a marker of sources of THS pollution in a child’s environment not captured by measurement of urinary cotinine alone. HN levels provide additional information about TSE, complementing other biomarkers when assessing children’s overall TSE.