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Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200242/ https://www.ncbi.nlm.nih.gov/pubmed/37038797 http://dx.doi.org/10.3233/THC-236039 |
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author | Wang, Yujiao Wang, Xiangxiang Jiang, Kailin Yang, Keming Ling, Jianghong |
author_facet | Wang, Yujiao Wang, Xiangxiang Jiang, Kailin Yang, Keming Ling, Jianghong |
author_sort | Wang, Yujiao |
collection | PubMed |
description | BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular targets and mechanisms in the treatment of FD. METHODS: Active compounds for CSP were identified from the TCMSP and SymMap databases, and the relevant targets were predicted. FD-related targets were obtained from the GeneCards and CTD database. In addition, using the protein-protein interactions (PPI) analysis, the common targets were obtained. Furthermore, the compound-target networks were created with Cytoscape. Finally, molecular docking was performed to identify the core targets and validate them experimentally. RESULTS: In total, 78 active compounds and 671 related targets of CSP were obtained. PPI network analysis identified 15 key FD-related compound targets. Molecular docking revealed that sitosterol and hyndarin exhibited good binding activities with AKT1 and IL6, respectively. Animal experiments have shown that CSP effectively increased the protein levels of AKT1 and reduced the serum levels of IL-6 in FD rats. CONCLUSION: This study provides a theoretical evidence for the analysis of the molecular targets and mechanisms of the action of CSP in FD. |
format | Online Article Text |
id | pubmed-10200242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102002422023-05-22 Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia Wang, Yujiao Wang, Xiangxiang Jiang, Kailin Yang, Keming Ling, Jianghong Technol Health Care Research Article BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular targets and mechanisms in the treatment of FD. METHODS: Active compounds for CSP were identified from the TCMSP and SymMap databases, and the relevant targets were predicted. FD-related targets were obtained from the GeneCards and CTD database. In addition, using the protein-protein interactions (PPI) analysis, the common targets were obtained. Furthermore, the compound-target networks were created with Cytoscape. Finally, molecular docking was performed to identify the core targets and validate them experimentally. RESULTS: In total, 78 active compounds and 671 related targets of CSP were obtained. PPI network analysis identified 15 key FD-related compound targets. Molecular docking revealed that sitosterol and hyndarin exhibited good binding activities with AKT1 and IL6, respectively. Animal experiments have shown that CSP effectively increased the protein levels of AKT1 and reduced the serum levels of IL-6 in FD rats. CONCLUSION: This study provides a theoretical evidence for the analysis of the molecular targets and mechanisms of the action of CSP in FD. IOS Press 2023-04-28 /pmc/articles/PMC10200242/ /pubmed/37038797 http://dx.doi.org/10.3233/THC-236039 Text en © 2023 – The authors. Published by IOS Press. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Yujiao Wang, Xiangxiang Jiang, Kailin Yang, Keming Ling, Jianghong Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title | Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title_full | Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title_fullStr | Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title_full_unstemmed | Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title_short | Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia |
title_sort | network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of chaihu shugan powder in the treatment of functional dyspepsia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200242/ https://www.ncbi.nlm.nih.gov/pubmed/37038797 http://dx.doi.org/10.3233/THC-236039 |
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