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Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia

BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular...

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Autores principales: Wang, Yujiao, Wang, Xiangxiang, Jiang, Kailin, Yang, Keming, Ling, Jianghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200242/
https://www.ncbi.nlm.nih.gov/pubmed/37038797
http://dx.doi.org/10.3233/THC-236039
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author Wang, Yujiao
Wang, Xiangxiang
Jiang, Kailin
Yang, Keming
Ling, Jianghong
author_facet Wang, Yujiao
Wang, Xiangxiang
Jiang, Kailin
Yang, Keming
Ling, Jianghong
author_sort Wang, Yujiao
collection PubMed
description BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular targets and mechanisms in the treatment of FD. METHODS: Active compounds for CSP were identified from the TCMSP and SymMap databases, and the relevant targets were predicted. FD-related targets were obtained from the GeneCards and CTD database. In addition, using the protein-protein interactions (PPI) analysis, the common targets were obtained. Furthermore, the compound-target networks were created with Cytoscape. Finally, molecular docking was performed to identify the core targets and validate them experimentally. RESULTS: In total, 78 active compounds and 671 related targets of CSP were obtained. PPI network analysis identified 15 key FD-related compound targets. Molecular docking revealed that sitosterol and hyndarin exhibited good binding activities with AKT1 and IL6, respectively. Animal experiments have shown that CSP effectively increased the protein levels of AKT1 and reduced the serum levels of IL-6 in FD rats. CONCLUSION: This study provides a theoretical evidence for the analysis of the molecular targets and mechanisms of the action of CSP in FD.
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spelling pubmed-102002422023-05-22 Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia Wang, Yujiao Wang, Xiangxiang Jiang, Kailin Yang, Keming Ling, Jianghong Technol Health Care Research Article BACKGROUND: Chaihu Shugan powder (CSP) is a prevalent prescription product used in the treatment functional dyspepsia (FD) in China. However, the underlying pharmacological mechanisms involved in the treatment of FD remain unclear. OBJECTIVE: To explore the key components of CSP and their molecular targets and mechanisms in the treatment of FD. METHODS: Active compounds for CSP were identified from the TCMSP and SymMap databases, and the relevant targets were predicted. FD-related targets were obtained from the GeneCards and CTD database. In addition, using the protein-protein interactions (PPI) analysis, the common targets were obtained. Furthermore, the compound-target networks were created with Cytoscape. Finally, molecular docking was performed to identify the core targets and validate them experimentally. RESULTS: In total, 78 active compounds and 671 related targets of CSP were obtained. PPI network analysis identified 15 key FD-related compound targets. Molecular docking revealed that sitosterol and hyndarin exhibited good binding activities with AKT1 and IL6, respectively. Animal experiments have shown that CSP effectively increased the protein levels of AKT1 and reduced the serum levels of IL-6 in FD rats. CONCLUSION: This study provides a theoretical evidence for the analysis of the molecular targets and mechanisms of the action of CSP in FD. IOS Press 2023-04-28 /pmc/articles/PMC10200242/ /pubmed/37038797 http://dx.doi.org/10.3233/THC-236039 Text en © 2023 – The authors. Published by IOS Press. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Yujiao
Wang, Xiangxiang
Jiang, Kailin
Yang, Keming
Ling, Jianghong
Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title_full Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title_fullStr Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title_full_unstemmed Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title_short Network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of Chaihu Shugan powder in the treatment of functional dyspepsia
title_sort network pharmacology and experimental studies for deciphering the molecular targets and mechanisms of chaihu shugan powder in the treatment of functional dyspepsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200242/
https://www.ncbi.nlm.nih.gov/pubmed/37038797
http://dx.doi.org/10.3233/THC-236039
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