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Spatial metabolomics for symbiotic marine invertebrates
Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, elimin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200813/ https://www.ncbi.nlm.nih.gov/pubmed/37202120 http://dx.doi.org/10.26508/lsa.202301900 |
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author | Chan, Wing Yan Rudd, David van Oppen, Madeleine JH |
author_facet | Chan, Wing Yan Rudd, David van Oppen, Madeleine JH |
author_sort | Chan, Wing Yan |
collection | PubMed |
description | Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, eliminating dimensionality and dampening analytical sensitivity. We have developed a mass spectrometry imaging workflow for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. The mass spectrometry imaging method provides critical functional insights that cannot be gleaned from bulk tissue analyses or other presently available spatial methods. We show that cnidarian hosts may regulate microalgal symbiont acquisition and rejection through specific ceramides distributed throughout the tissue lining the gastrovascular cavity. The distribution pattern of betaine lipids showed that once resident, symbionts primarily reside in light-exposed tentacles to generate photosynthate. Spatial patterns of these metabolites also revealed that symbiont identity can drive host metabolism. |
format | Online Article Text |
id | pubmed-10200813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-102008132023-05-23 Spatial metabolomics for symbiotic marine invertebrates Chan, Wing Yan Rudd, David van Oppen, Madeleine JH Life Sci Alliance Methods Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, eliminating dimensionality and dampening analytical sensitivity. We have developed a mass spectrometry imaging workflow for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. The mass spectrometry imaging method provides critical functional insights that cannot be gleaned from bulk tissue analyses or other presently available spatial methods. We show that cnidarian hosts may regulate microalgal symbiont acquisition and rejection through specific ceramides distributed throughout the tissue lining the gastrovascular cavity. The distribution pattern of betaine lipids showed that once resident, symbionts primarily reside in light-exposed tentacles to generate photosynthate. Spatial patterns of these metabolites also revealed that symbiont identity can drive host metabolism. Life Science Alliance LLC 2023-05-18 /pmc/articles/PMC10200813/ /pubmed/37202120 http://dx.doi.org/10.26508/lsa.202301900 Text en © 2023 Chan et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Methods Chan, Wing Yan Rudd, David van Oppen, Madeleine JH Spatial metabolomics for symbiotic marine invertebrates |
title | Spatial metabolomics for symbiotic marine invertebrates |
title_full | Spatial metabolomics for symbiotic marine invertebrates |
title_fullStr | Spatial metabolomics for symbiotic marine invertebrates |
title_full_unstemmed | Spatial metabolomics for symbiotic marine invertebrates |
title_short | Spatial metabolomics for symbiotic marine invertebrates |
title_sort | spatial metabolomics for symbiotic marine invertebrates |
topic | Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200813/ https://www.ncbi.nlm.nih.gov/pubmed/37202120 http://dx.doi.org/10.26508/lsa.202301900 |
work_keys_str_mv | AT chanwingyan spatialmetabolomicsforsymbioticmarineinvertebrates AT rudddavid spatialmetabolomicsforsymbioticmarineinvertebrates AT vanoppenmadeleinejh spatialmetabolomicsforsymbioticmarineinvertebrates |