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Spatial metabolomics for symbiotic marine invertebrates

Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, elimin...

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Autores principales: Chan, Wing Yan, Rudd, David, van Oppen, Madeleine JH
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200813/
https://www.ncbi.nlm.nih.gov/pubmed/37202120
http://dx.doi.org/10.26508/lsa.202301900
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author Chan, Wing Yan
Rudd, David
van Oppen, Madeleine JH
author_facet Chan, Wing Yan
Rudd, David
van Oppen, Madeleine JH
author_sort Chan, Wing Yan
collection PubMed
description Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, eliminating dimensionality and dampening analytical sensitivity. We have developed a mass spectrometry imaging workflow for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. The mass spectrometry imaging method provides critical functional insights that cannot be gleaned from bulk tissue analyses or other presently available spatial methods. We show that cnidarian hosts may regulate microalgal symbiont acquisition and rejection through specific ceramides distributed throughout the tissue lining the gastrovascular cavity. The distribution pattern of betaine lipids showed that once resident, symbionts primarily reside in light-exposed tentacles to generate photosynthate. Spatial patterns of these metabolites also revealed that symbiont identity can drive host metabolism.
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spelling pubmed-102008132023-05-23 Spatial metabolomics for symbiotic marine invertebrates Chan, Wing Yan Rudd, David van Oppen, Madeleine JH Life Sci Alliance Methods Microbial symbionts frequently localize within specific body structures or cell types of their multicellular hosts. This spatiotemporal niche is critical to host health, nutrient exchange, and fitness. Measuring host–microbe metabolite exchange has conventionally relied on tissue homogenates, eliminating dimensionality and dampening analytical sensitivity. We have developed a mass spectrometry imaging workflow for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. The mass spectrometry imaging method provides critical functional insights that cannot be gleaned from bulk tissue analyses or other presently available spatial methods. We show that cnidarian hosts may regulate microalgal symbiont acquisition and rejection through specific ceramides distributed throughout the tissue lining the gastrovascular cavity. The distribution pattern of betaine lipids showed that once resident, symbionts primarily reside in light-exposed tentacles to generate photosynthate. Spatial patterns of these metabolites also revealed that symbiont identity can drive host metabolism. Life Science Alliance LLC 2023-05-18 /pmc/articles/PMC10200813/ /pubmed/37202120 http://dx.doi.org/10.26508/lsa.202301900 Text en © 2023 Chan et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Methods
Chan, Wing Yan
Rudd, David
van Oppen, Madeleine JH
Spatial metabolomics for symbiotic marine invertebrates
title Spatial metabolomics for symbiotic marine invertebrates
title_full Spatial metabolomics for symbiotic marine invertebrates
title_fullStr Spatial metabolomics for symbiotic marine invertebrates
title_full_unstemmed Spatial metabolomics for symbiotic marine invertebrates
title_short Spatial metabolomics for symbiotic marine invertebrates
title_sort spatial metabolomics for symbiotic marine invertebrates
topic Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200813/
https://www.ncbi.nlm.nih.gov/pubmed/37202120
http://dx.doi.org/10.26508/lsa.202301900
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