Comparative analyses define differences between BHD-associated renal tumour and sporadic chromophobe renal cell carcinoma

BACKGROUND: Birt-Hogg-Dubé (BHD) syndrome, caused by germline alteration of folliculin (FLCN) gene, develops hybrid oncocytic/chromophobe tumour (HOCT) and chromophobe renal cell carcinoma (ChRCC), whereas sporadic ChRCC does not harbor FLCN alteration. To date, molecular characteristics of these si...

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Detalles Bibliográficos
Autores principales: Jikuya, Ryosuke, Johnson, Todd A., Maejima, Kazuhiro, An, Jisong, Ju, Young-Seok, Lee, Hwajin, Ha, Kyungsik, Song, WooJeung, Kim, Youngwook, Okawa, Yuki, Sasagawa, Shota, Kanazashi, Yuki, Fujita, Masashi, Imoto, Seiya, Mitome, Taku, Ohtake, Shinji, Noguchi, Go, Kawaura, Sachi, Iribe, Yasuhiro, Aomori, Kota, Tatenuma, Tomoyuki, Komeya, Mitsuru, Ito, Hiroki, Ito, Yusuke, Muraoka, Kentaro, Furuya, Mitsuko, Kato, Ikuma, Fujii, Satoshi, Hamanoue, Haruka, Tamura, Tomohiko, Baba, Masaya, Suda, Toshio, Kodama, Tatsuhiko, Makiyama, Kazuhide, Yao, Masahiro, Shuch, Brian M., Ricketts, Christopher J., Schmidt, Laura S., Linehan, W. Marston, Nakagawa, Hidewaki, Hasumi, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200853/
https://www.ncbi.nlm.nih.gov/pubmed/37182269
http://dx.doi.org/10.1016/j.ebiom.2023.104596
Descripción
Sumario:BACKGROUND: Birt-Hogg-Dubé (BHD) syndrome, caused by germline alteration of folliculin (FLCN) gene, develops hybrid oncocytic/chromophobe tumour (HOCT) and chromophobe renal cell carcinoma (ChRCC), whereas sporadic ChRCC does not harbor FLCN alteration. To date, molecular characteristics of these similar histological types of tumours have been incompletely elucidated. METHODS: To elucidate renal tumourigenesis of BHD-associated renal tumours and sporadic renal tumours, we conducted whole genome sequencing (WGS) and RNA-sequencing (RNA-seq) of sixteen BHD-associated renal tumours from nine unrelated BHD patients, twenty-one sporadic ChRCCs and seven sporadic oncocytomas. We then compared somatic mutation profiles with FLCN variants and RNA expression profiles between BHD-associated renal tumours and sporadic renal tumours. FINDINGS: RNA-seq analysis revealed that BHD-associated renal tumours and sporadic renal tumours have totally different expression profiles. Sporadic ChRCCs were clustered into two distinct clusters characterized by L1CAM and FOXI1 expressions, molecular markers for renal tubule subclasses. Increased mitochondrial DNA (mtDNA) copy number with fewer variants was observed in BHD-associated renal tumours compared to sporadic ChRCCs. Cell-of-origin analysis using WGS data demonstrated that BHD-associated renal tumours and sporadic ChRCCs may arise from different cells of origin and second hit FLCN alterations may occur in early third decade of life in BHD patients. INTERPRETATION: These data further our understanding of renal tumourigenesis of these two different types of renal tumours with similar histology. FUNDING: This study was supported by 10.13039/501100001691JSPS KAKENHI Grants, 10.13039/501100006264RIKEN internal grant, and the Intramural Research Program of the 10.13039/100000002National Institutes of Health (10.13039/100000002NIH), 10.13039/100000054National Cancer Institute (NCI), Center for Cancer Research.

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