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Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice

Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how...

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Autores principales: Wang, Fei, Wang, Yan, Ying, Luying, Lu, Hong, Liu, Yijian, Liu, Yu, Xu, Jiming, Wu, Yunrong, Mo, Xiaorong, Wu, Zhongchang, Mao, Chuanzao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200874/
https://www.ncbi.nlm.nih.gov/pubmed/37223782
http://dx.doi.org/10.3389/fpls.2023.1164441
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author Wang, Fei
Wang, Yan
Ying, Luying
Lu, Hong
Liu, Yijian
Liu, Yu
Xu, Jiming
Wu, Yunrong
Mo, Xiaorong
Wu, Zhongchang
Mao, Chuanzao
author_facet Wang, Fei
Wang, Yan
Ying, Luying
Lu, Hong
Liu, Yijian
Liu, Yu
Xu, Jiming
Wu, Yunrong
Mo, Xiaorong
Wu, Zhongchang
Mao, Chuanzao
author_sort Wang, Fei
collection PubMed
description Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice (Oryza sativa) to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes PT2 and SPX1 and repressed the N responsive genes NLP1 and NRT2.1. These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses.
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spelling pubmed-102008742023-05-23 Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice Wang, Fei Wang, Yan Ying, Luying Lu, Hong Liu, Yijian Liu, Yu Xu, Jiming Wu, Yunrong Mo, Xiaorong Wu, Zhongchang Mao, Chuanzao Front Plant Sci Plant Science Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice (Oryza sativa) to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes PT2 and SPX1 and repressed the N responsive genes NLP1 and NRT2.1. These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses. Frontiers Media S.A. 2023-05-08 /pmc/articles/PMC10200874/ /pubmed/37223782 http://dx.doi.org/10.3389/fpls.2023.1164441 Text en Copyright © 2023 Wang, Wang, Ying, Lu, Liu, Liu, Xu, Wu, Mo, Wu and Mao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Fei
Wang, Yan
Ying, Luying
Lu, Hong
Liu, Yijian
Liu, Yu
Xu, Jiming
Wu, Yunrong
Mo, Xiaorong
Wu, Zhongchang
Mao, Chuanzao
Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title_full Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title_fullStr Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title_full_unstemmed Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title_short Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
title_sort integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200874/
https://www.ncbi.nlm.nih.gov/pubmed/37223782
http://dx.doi.org/10.3389/fpls.2023.1164441
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