Binding to the conserved and stably folded guide RNA pseudoknot induces Cas12a conformational changes during ribonucleoprotein assembly

Ribonucleoproteins (RNPs) comprise one or more RNA and protein molecules that interact to form a stable complex, which commonly involves conformational changes in the more flexible RNA components. Here, we propose that Cas12a RNP assembly with its cognate CRISPR RNA (crRNA) guide instead proceeds pr...

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Detalles Bibliográficos
Autores principales: Sudhakar, Sruthi, Barkau, Christopher L., Chilamkurthy, Ramadevi, Barber, Halle M., Pater, Adrian A., Moran, Sean D., Damha, Masad J., Pradeepkumar, P.I., Gagnon, Keith T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200996/
https://www.ncbi.nlm.nih.gov/pubmed/37059184
http://dx.doi.org/10.1016/j.jbc.2023.104700

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