Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions

Fetal extravillous trophoblasts (EVTs) are the most invasive cells of the placenta and play a key role in modulating maternal immune responses. Here, we present a protocol to purify and culture human leukocyte antigen-G (HLA-G)+ EVTs. We describe steps for tissue dissection, tissue digestion, densit...

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Detalles Bibliográficos
Autores principales: Hamilton, Ira, Ikumi, Nadia M., Kshirsagar, Sarika, Goodman, Wendy A., Tilburgs, Tamara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10200998/
https://www.ncbi.nlm.nih.gov/pubmed/37178111
http://dx.doi.org/10.1016/j.xpro.2023.102276

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