Rolling circle extension-assisted loop-mediated isothermal amplification (Rol-LAMP) method for locus-specific and visible detection of RNA N(6)-methyladenosine

N6-methyladenosine (m(6)A) is the most prevalent RNA modification in eukaryotic mRNAs. Currently available detection methods for locus-specific m(6)A marks rely on RT-qPCR, radioactive methods, or high-throughput sequencing. Here, we develop a non-qPCR, ultrasensitive, isothermal, and naked-eye visi...

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Detalles Bibliográficos
Autores principales: Li, Jiexin, Zhou, Jiawang, Xia, Yan, Rui, Yalan, Yang, Xianyuan, Xie, Guoyou, Jiang, Guanmin, Wang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10201442/
https://www.ncbi.nlm.nih.gov/pubmed/36971119
http://dx.doi.org/10.1093/nar/gkad200

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