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Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes

The liver is the hub of human metabolism and involves many diseases. To better work on the mechanism and treatment of liver diseases, it is of particular interest to design 3-dimensional scaffolds suitable for culturing hepatocytes in vitro to simulate their metabolic and regenerative abilities. In...

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Autores principales: Li, Xinmeng, Ding, Weixiao, Wang, Shujun, Yang, Luyu, Yu, Qingqing, Xiao, Changji, Chen, Guangbo, Zhang, Lei, Guan, Shanyue, Sun, Dongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202184/
https://www.ncbi.nlm.nih.gov/pubmed/37223548
http://dx.doi.org/10.34133/cbsystems.0021
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author Li, Xinmeng
Ding, Weixiao
Wang, Shujun
Yang, Luyu
Yu, Qingqing
Xiao, Changji
Chen, Guangbo
Zhang, Lei
Guan, Shanyue
Sun, Dongping
author_facet Li, Xinmeng
Ding, Weixiao
Wang, Shujun
Yang, Luyu
Yu, Qingqing
Xiao, Changji
Chen, Guangbo
Zhang, Lei
Guan, Shanyue
Sun, Dongping
author_sort Li, Xinmeng
collection PubMed
description The liver is the hub of human metabolism and involves many diseases. To better work on the mechanism and treatment of liver diseases, it is of particular interest to design 3-dimensional scaffolds suitable for culturing hepatocytes in vitro to simulate their metabolic and regenerative abilities. In this study, sulfated bacterial cellulose (SBC) was prepared as the building block of cell scaffolds, motivated by the anionic nature and 3-dimensional structure of hepatic extracellular matrix, and its reaction condition for sulfate esterification was optimized by changing the reaction time. The analysis and study of the microscopic morphology, structure, and cytocompatibility of SBCs showed that they possess good biocompatibility and meet the requirements for tissue engineering. Next, SBC was mixed with gelatin for composite scaffolds (SBC/Gel) for culturing hepatocytes by homogenization and freeze-drying methods, whose physical properties such as pore size, porosity, and compression properties were compared with gelatin (Gel) scaffolds as the control group, and the cytological activity and hemocompatibility of the composite scaffolds were investigated. The results showed that the SBC/Gel composite has better porosity and compression properties, as well as good cytocompatibility and hemocompatibility, and could be applied to 3-dimensional culture of hepatocytes for drug screening or liver tissue engineering.
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spelling pubmed-102021842023-05-23 Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes Li, Xinmeng Ding, Weixiao Wang, Shujun Yang, Luyu Yu, Qingqing Xiao, Changji Chen, Guangbo Zhang, Lei Guan, Shanyue Sun, Dongping Cyborg Bionic Syst Research Article The liver is the hub of human metabolism and involves many diseases. To better work on the mechanism and treatment of liver diseases, it is of particular interest to design 3-dimensional scaffolds suitable for culturing hepatocytes in vitro to simulate their metabolic and regenerative abilities. In this study, sulfated bacterial cellulose (SBC) was prepared as the building block of cell scaffolds, motivated by the anionic nature and 3-dimensional structure of hepatic extracellular matrix, and its reaction condition for sulfate esterification was optimized by changing the reaction time. The analysis and study of the microscopic morphology, structure, and cytocompatibility of SBCs showed that they possess good biocompatibility and meet the requirements for tissue engineering. Next, SBC was mixed with gelatin for composite scaffolds (SBC/Gel) for culturing hepatocytes by homogenization and freeze-drying methods, whose physical properties such as pore size, porosity, and compression properties were compared with gelatin (Gel) scaffolds as the control group, and the cytological activity and hemocompatibility of the composite scaffolds were investigated. The results showed that the SBC/Gel composite has better porosity and compression properties, as well as good cytocompatibility and hemocompatibility, and could be applied to 3-dimensional culture of hepatocytes for drug screening or liver tissue engineering. AAAS 2023-04-12 /pmc/articles/PMC10202184/ /pubmed/37223548 http://dx.doi.org/10.34133/cbsystems.0021 Text en Copyright © 2023 Xinmeng Li et al. https://creativecommons.org/licenses/by/4.0/Exclusive licensee Beijing Institute of Technology Press. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Li, Xinmeng
Ding, Weixiao
Wang, Shujun
Yang, Luyu
Yu, Qingqing
Xiao, Changji
Chen, Guangbo
Zhang, Lei
Guan, Shanyue
Sun, Dongping
Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title_full Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title_fullStr Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title_full_unstemmed Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title_short Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes
title_sort three-dimensional sulfated bacterial cellulose/gelatin composite scaffolds for culturing hepatocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202184/
https://www.ncbi.nlm.nih.gov/pubmed/37223548
http://dx.doi.org/10.34133/cbsystems.0021
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