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Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons

Adult-born cells, arriving daily into the rodent olfactory bulb, either integrate into the neural circuitry or get eliminated. However, whether these two populations differ in their morphological or functional properties remains unclear. Using longitudinal in vivo two-photon imaging, we monitored de...

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Detalles Bibliográficos
Autores principales: Su, Xin, Kovalchuk, Yury, Mojtahedi, Nima, Kamari, Farzin, Claassen, Manfred, Garaschuk, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202693/
https://www.ncbi.nlm.nih.gov/pubmed/37116486
http://dx.doi.org/10.1016/j.stemcr.2023.03.018
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author Su, Xin
Kovalchuk, Yury
Mojtahedi, Nima
Kamari, Farzin
Claassen, Manfred
Garaschuk, Olga
author_facet Su, Xin
Kovalchuk, Yury
Mojtahedi, Nima
Kamari, Farzin
Claassen, Manfred
Garaschuk, Olga
author_sort Su, Xin
collection PubMed
description Adult-born cells, arriving daily into the rodent olfactory bulb, either integrate into the neural circuitry or get eliminated. However, whether these two populations differ in their morphological or functional properties remains unclear. Using longitudinal in vivo two-photon imaging, we monitored dendritic morphogenesis, odor-evoked responsiveness, ongoing Ca(2+) signaling, and survival/death of adult-born juxtaglomerular neurons (abJGNs). We found that the maturation of abJGNs is accompanied by a significant reduction in dendritic complexity, with surviving and subsequently eliminated cells showing similar degrees of dendritic remodeling. Surprisingly, ∼63% of eliminated abJGNs acquired odor responsiveness before death, with amplitudes and time courses of odor-evoked responses similar to those recorded in surviving cells. However, the subsequently eliminated cell population exhibited significantly higher ongoing Ca(2+) signals, with a difference visible even 10 days before death. Quantitative supervised machine learning analysis revealed a relationship between the abJGNs’ activity and survival probability, with low neuronal activity being supportive for survival.
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spelling pubmed-102026932023-05-24 Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons Su, Xin Kovalchuk, Yury Mojtahedi, Nima Kamari, Farzin Claassen, Manfred Garaschuk, Olga Stem Cell Reports Article Adult-born cells, arriving daily into the rodent olfactory bulb, either integrate into the neural circuitry or get eliminated. However, whether these two populations differ in their morphological or functional properties remains unclear. Using longitudinal in vivo two-photon imaging, we monitored dendritic morphogenesis, odor-evoked responsiveness, ongoing Ca(2+) signaling, and survival/death of adult-born juxtaglomerular neurons (abJGNs). We found that the maturation of abJGNs is accompanied by a significant reduction in dendritic complexity, with surviving and subsequently eliminated cells showing similar degrees of dendritic remodeling. Surprisingly, ∼63% of eliminated abJGNs acquired odor responsiveness before death, with amplitudes and time courses of odor-evoked responses similar to those recorded in surviving cells. However, the subsequently eliminated cell population exhibited significantly higher ongoing Ca(2+) signals, with a difference visible even 10 days before death. Quantitative supervised machine learning analysis revealed a relationship between the abJGNs’ activity and survival probability, with low neuronal activity being supportive for survival. Elsevier 2023-04-27 /pmc/articles/PMC10202693/ /pubmed/37116486 http://dx.doi.org/10.1016/j.stemcr.2023.03.018 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Su, Xin
Kovalchuk, Yury
Mojtahedi, Nima
Kamari, Farzin
Claassen, Manfred
Garaschuk, Olga
Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title_full Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title_fullStr Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title_full_unstemmed Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title_short Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
title_sort neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202693/
https://www.ncbi.nlm.nih.gov/pubmed/37116486
http://dx.doi.org/10.1016/j.stemcr.2023.03.018
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