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Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice

Amyloid A (AA) amyloidosis is experimentally transmissible in some animal species, such as mice and chickens. While the spleen is important as the initial deposition site in the transmission of AA amyloidosis, it is not essential for establishing the transmission, and its role is not precisely under...

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Autores principales: Iwaide, Susumu, Oba, Ryohei, Kobayashi, Natsumi, Murakami, Tomoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202707/
https://www.ncbi.nlm.nih.gov/pubmed/36418077
http://dx.doi.org/10.1538/expanim.22-0125
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author Iwaide, Susumu
Oba, Ryohei
Kobayashi, Natsumi
Murakami, Tomoaki
author_facet Iwaide, Susumu
Oba, Ryohei
Kobayashi, Natsumi
Murakami, Tomoaki
author_sort Iwaide, Susumu
collection PubMed
description Amyloid A (AA) amyloidosis is experimentally transmissible in some animal species, such as mice and chickens. While the spleen is important as the initial deposition site in the transmission of AA amyloidosis, it is not essential for establishing the transmission, and its role is not precisely understood. In this study, to clarify why the spleen is the first site of deposition in transmissible AA amyloidosis, we administered amyloid enhancing factor, which is AA fibrils extracted from AA amyloidosis affected mouse to local organs (liver, spleen, kidney, stomach wall, and Peyer’s patches), to tail vein and into peritoneum; then compared the amyloid distribution. Interestingly, initial amyloid deposition was observed at the administration site in each administered organ, not just the spleen. Furthermore, the amount of amyloid deposition in intra-organ administration groups was larger than that of the intravenous or intraperitoneal administration groups. This study indicates that locally exposed AEF initiates in situ amyloid deposition, from which amyloid deposition spreads throughout the body.
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spelling pubmed-102027072023-05-24 Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice Iwaide, Susumu Oba, Ryohei Kobayashi, Natsumi Murakami, Tomoaki Exp Anim Original Amyloid A (AA) amyloidosis is experimentally transmissible in some animal species, such as mice and chickens. While the spleen is important as the initial deposition site in the transmission of AA amyloidosis, it is not essential for establishing the transmission, and its role is not precisely understood. In this study, to clarify why the spleen is the first site of deposition in transmissible AA amyloidosis, we administered amyloid enhancing factor, which is AA fibrils extracted from AA amyloidosis affected mouse to local organs (liver, spleen, kidney, stomach wall, and Peyer’s patches), to tail vein and into peritoneum; then compared the amyloid distribution. Interestingly, initial amyloid deposition was observed at the administration site in each administered organ, not just the spleen. Furthermore, the amount of amyloid deposition in intra-organ administration groups was larger than that of the intravenous or intraperitoneal administration groups. This study indicates that locally exposed AEF initiates in situ amyloid deposition, from which amyloid deposition spreads throughout the body. Japanese Association for Laboratory Animal Science 2022-11-23 2023 /pmc/articles/PMC10202707/ /pubmed/36418077 http://dx.doi.org/10.1538/expanim.22-0125 Text en ©2023 Japanese Association for Laboratory Animal Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Iwaide, Susumu
Oba, Ryohei
Kobayashi, Natsumi
Murakami, Tomoaki
Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title_full Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title_fullStr Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title_full_unstemmed Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title_short Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
title_sort local administration of amyloid enhancing factor initiates in situ amyloid a deposition followed by systemic lesions in mice
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202707/
https://www.ncbi.nlm.nih.gov/pubmed/36418077
http://dx.doi.org/10.1538/expanim.22-0125
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