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A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke

Ischemic stroke (IS), resulting from the occlusion of the cerebral artery and subsequent interruption of blood flow, represents a major and critical threat to public health. Oxidative stress (OS) has been confirmed to play a role in the IS pathological process and neural death. Understanding the ess...

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Autores principales: Zhou, Qing, Dong, Yang, Wang, Kun, Wang, Ziyan, Ma, Bingquan, Yang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10203175/
https://www.ncbi.nlm.nih.gov/pubmed/37229425
http://dx.doi.org/10.3389/fnins.2023.1166010
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author Zhou, Qing
Dong, Yang
Wang, Kun
Wang, Ziyan
Ma, Bingquan
Yang, Bo
author_facet Zhou, Qing
Dong, Yang
Wang, Kun
Wang, Ziyan
Ma, Bingquan
Yang, Bo
author_sort Zhou, Qing
collection PubMed
description Ischemic stroke (IS), resulting from the occlusion of the cerebral artery and subsequent interruption of blood flow, represents a major and critical threat to public health. Oxidative stress (OS) has been confirmed to play a role in the IS pathological process and neural death. Understanding the essential role of OS-related genes in ischemic stroke is critical to understanding the current perception of the pathophysiological process in IS. Herein, by integrating three IS datasets (GSE16561, GSE22255, and GSE58294), we divided IS samples into the low- and high-OS groups by calculating the OS score identified by the oxidative stress gene set. The functional enrichment analysis of differentially expressed genes (DEGs) between the low- and high-OS groups indicated that DEGs were associated with hypoxia, the inflammatory response, and oxidative phosphorylation pathways. Furthermore, nine hub genes (namely TLR1, CXCL1, MMP9, TLR4, IL1R2, EGR1, FOS, CXCL10, and DUSP1) were identified through the Girvan–Newman algorithm and cytoHubba algorithms. Nine hub genes were highly expressed in IS samples and positively related to neutrophils and macrophages. Drug-sensitive analysis targeting hub genes defined allopurinol and nickel sulfate as potential candidates for impairing the neural death caused by oxidative stress in IS. Finally, we employed five machine learning methods to check the efficacy of the predictive model identified by nine hub genes. The results showed that our model had superior power for predicting the OS activity of IS patients. TLR4 was found to have excellent diagnostic value and a wide-spectrum interaction with other hub genes. Our research emphasized the impact of oxidative stress on ischemic stroke, which supports the idea that antioxidants hold great promise in ischemic stroke therapy.
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spelling pubmed-102031752023-05-24 A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke Zhou, Qing Dong, Yang Wang, Kun Wang, Ziyan Ma, Bingquan Yang, Bo Front Neurosci Neuroscience Ischemic stroke (IS), resulting from the occlusion of the cerebral artery and subsequent interruption of blood flow, represents a major and critical threat to public health. Oxidative stress (OS) has been confirmed to play a role in the IS pathological process and neural death. Understanding the essential role of OS-related genes in ischemic stroke is critical to understanding the current perception of the pathophysiological process in IS. Herein, by integrating three IS datasets (GSE16561, GSE22255, and GSE58294), we divided IS samples into the low- and high-OS groups by calculating the OS score identified by the oxidative stress gene set. The functional enrichment analysis of differentially expressed genes (DEGs) between the low- and high-OS groups indicated that DEGs were associated with hypoxia, the inflammatory response, and oxidative phosphorylation pathways. Furthermore, nine hub genes (namely TLR1, CXCL1, MMP9, TLR4, IL1R2, EGR1, FOS, CXCL10, and DUSP1) were identified through the Girvan–Newman algorithm and cytoHubba algorithms. Nine hub genes were highly expressed in IS samples and positively related to neutrophils and macrophages. Drug-sensitive analysis targeting hub genes defined allopurinol and nickel sulfate as potential candidates for impairing the neural death caused by oxidative stress in IS. Finally, we employed five machine learning methods to check the efficacy of the predictive model identified by nine hub genes. The results showed that our model had superior power for predicting the OS activity of IS patients. TLR4 was found to have excellent diagnostic value and a wide-spectrum interaction with other hub genes. Our research emphasized the impact of oxidative stress on ischemic stroke, which supports the idea that antioxidants hold great promise in ischemic stroke therapy. Frontiers Media S.A. 2023-05-09 /pmc/articles/PMC10203175/ /pubmed/37229425 http://dx.doi.org/10.3389/fnins.2023.1166010 Text en Copyright © 2023 Zhou, Dong, Wang, Wang, Ma and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Zhou, Qing
Dong, Yang
Wang, Kun
Wang, Ziyan
Ma, Bingquan
Yang, Bo
A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title_full A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title_fullStr A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title_full_unstemmed A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title_short A comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
title_sort comprehensive analysis of the hub genes for oxidative stress in ischemic stroke
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10203175/
https://www.ncbi.nlm.nih.gov/pubmed/37229425
http://dx.doi.org/10.3389/fnins.2023.1166010
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