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Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide. Due to the possibility of asymptomatic carriers, late diagnosis of infected individuals can lead to uncontrollable transmission of the disea...

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Autores principales: Moshref, Zahra Sadat, Jalali, Tahmineh, Rezaei Adriani, Razieh, Soltati, Elahe, Mousavi Gargari, Seyed Latif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204341/
https://www.ncbi.nlm.nih.gov/pubmed/37251485
http://dx.doi.org/10.1016/j.heliyon.2023.e16458
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author Moshref, Zahra Sadat
Jalali, Tahmineh
Rezaei Adriani, Razieh
Soltati, Elahe
Mousavi Gargari, Seyed Latif
author_facet Moshref, Zahra Sadat
Jalali, Tahmineh
Rezaei Adriani, Razieh
Soltati, Elahe
Mousavi Gargari, Seyed Latif
author_sort Moshref, Zahra Sadat
collection PubMed
description The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide. Due to the possibility of asymptomatic carriers, late diagnosis of infected individuals can lead to uncontrollable transmission of the disease, making early and accurate detection crucial in controlling the spread of the virus. In this study we identified high-binding-affinity aptamers targeting various strains of the SARS-CoV2 (COVID-19) virus, using the GO-Cell-SELEX (Graphene Oxide- Systematic Evolution of Ligands by Exponential Enrichment) strategy. A total of 96 aptamers were developed through 11 rounds of GO-Cell-SELEX from a random 40 nucleotide single-strand DNA (ssDNA) aptamer library. Using the surface plasmon resonance (SPR) method, the dissociation constant (Kd) values of all aptamers were calculated and two aptamers 52 and 91 with Kd 50 and 61 were selected for enzyme-linked apta-sorbent assay (ELASA). Aptamer 91 could detect various strains of the virus in above 97% of clinical samples obtained from nasopharyngeal swaps (NPS) specimens kept in viral transport media (VTM), confirmed by real-time PCR assay at COVID-19 Reference Diagnostic Laboratory of Iran, Pasture Institute. Aptamer 52 could detect the SARS-CoV2 virus in a competitive lateral flow assay (LFA) to be considered for a future designed kit. These two simple, specific, and sensitive tests can be used in combination for rapid and early diagnosis of various strains of the COVID-19 virus. Our results suggest that these two discovered aptamers present an opportunity for developing a new rapid aptamer-based coronavirus diagnostic kit.
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spelling pubmed-102043412023-05-23 Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens Moshref, Zahra Sadat Jalali, Tahmineh Rezaei Adriani, Razieh Soltati, Elahe Mousavi Gargari, Seyed Latif Heliyon Research Article The emergence of the SARS-CoV-2 virus, an unknown strain of coronavirus, has resulted in severe acute respiratory syndrome with high mortality rates worldwide. Due to the possibility of asymptomatic carriers, late diagnosis of infected individuals can lead to uncontrollable transmission of the disease, making early and accurate detection crucial in controlling the spread of the virus. In this study we identified high-binding-affinity aptamers targeting various strains of the SARS-CoV2 (COVID-19) virus, using the GO-Cell-SELEX (Graphene Oxide- Systematic Evolution of Ligands by Exponential Enrichment) strategy. A total of 96 aptamers were developed through 11 rounds of GO-Cell-SELEX from a random 40 nucleotide single-strand DNA (ssDNA) aptamer library. Using the surface plasmon resonance (SPR) method, the dissociation constant (Kd) values of all aptamers were calculated and two aptamers 52 and 91 with Kd 50 and 61 were selected for enzyme-linked apta-sorbent assay (ELASA). Aptamer 91 could detect various strains of the virus in above 97% of clinical samples obtained from nasopharyngeal swaps (NPS) specimens kept in viral transport media (VTM), confirmed by real-time PCR assay at COVID-19 Reference Diagnostic Laboratory of Iran, Pasture Institute. Aptamer 52 could detect the SARS-CoV2 virus in a competitive lateral flow assay (LFA) to be considered for a future designed kit. These two simple, specific, and sensitive tests can be used in combination for rapid and early diagnosis of various strains of the COVID-19 virus. Our results suggest that these two discovered aptamers present an opportunity for developing a new rapid aptamer-based coronavirus diagnostic kit. Elsevier 2023-05-23 /pmc/articles/PMC10204341/ /pubmed/37251485 http://dx.doi.org/10.1016/j.heliyon.2023.e16458 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Moshref, Zahra Sadat
Jalali, Tahmineh
Rezaei Adriani, Razieh
Soltati, Elahe
Mousavi Gargari, Seyed Latif
Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title_full Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title_fullStr Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title_full_unstemmed Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title_short Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens
title_sort aptamer-based diagnosis of various sars-cov2 strains isolated from clinical specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204341/
https://www.ncbi.nlm.nih.gov/pubmed/37251485
http://dx.doi.org/10.1016/j.heliyon.2023.e16458
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