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Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle

Molecular detection of SARS-CoV-2 in gargle and saliva complements the standard analysis of nasopharyngeal swabs (NPS) specimens. Although gargle and saliva specimens can be readily obtained non-invasively, appropriate collection and processing of gargle and saliva specimens are critical to the accu...

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Autores principales: Liu, Yanming, Kumblathan, Teresa, Tao, Jeffrey, Xu, Jingyang, Feng, Wei, Xiao, Huyan, Hu, Jianyu, Huang, Camille V., Wu, Yiping, Zhang, Hongquan, Li, Xing-Fang, Le, X. Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204347/
https://www.ncbi.nlm.nih.gov/pubmed/37317683
http://dx.doi.org/10.1016/j.trac.2023.117107
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author Liu, Yanming
Kumblathan, Teresa
Tao, Jeffrey
Xu, Jingyang
Feng, Wei
Xiao, Huyan
Hu, Jianyu
Huang, Camille V.
Wu, Yiping
Zhang, Hongquan
Li, Xing-Fang
Le, X. Chris
author_facet Liu, Yanming
Kumblathan, Teresa
Tao, Jeffrey
Xu, Jingyang
Feng, Wei
Xiao, Huyan
Hu, Jianyu
Huang, Camille V.
Wu, Yiping
Zhang, Hongquan
Li, Xing-Fang
Le, X. Chris
author_sort Liu, Yanming
collection PubMed
description Molecular detection of SARS-CoV-2 in gargle and saliva complements the standard analysis of nasopharyngeal swabs (NPS) specimens. Although gargle and saliva specimens can be readily obtained non-invasively, appropriate collection and processing of gargle and saliva specimens are critical to the accuracy and sensitivity of the overall analytical method. This review highlights challenges and recent advances in the treatment of gargle and saliva samples for subsequent analysis using reverse transcription polymerase chain reaction (RT-PCR) and isothermal amplification techniques. Important considerations include appropriate collection of gargle and saliva samples, on-site inactivation of viruses in the sample, preservation of viral RNA, extraction and concentration of viral RNA, removal of substances that inhibit nucleic acid amplification reactions, and the compatibility of sample treatment protocols with the subsequent nucleic acid amplification and detection techniques. The principles and approaches discussed in this review are applicable to molecular detection of other microbial pathogens.
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spelling pubmed-102043472023-05-23 Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle Liu, Yanming Kumblathan, Teresa Tao, Jeffrey Xu, Jingyang Feng, Wei Xiao, Huyan Hu, Jianyu Huang, Camille V. Wu, Yiping Zhang, Hongquan Li, Xing-Fang Le, X. Chris Trends Analyt Chem Article Molecular detection of SARS-CoV-2 in gargle and saliva complements the standard analysis of nasopharyngeal swabs (NPS) specimens. Although gargle and saliva specimens can be readily obtained non-invasively, appropriate collection and processing of gargle and saliva specimens are critical to the accuracy and sensitivity of the overall analytical method. This review highlights challenges and recent advances in the treatment of gargle and saliva samples for subsequent analysis using reverse transcription polymerase chain reaction (RT-PCR) and isothermal amplification techniques. Important considerations include appropriate collection of gargle and saliva samples, on-site inactivation of viruses in the sample, preservation of viral RNA, extraction and concentration of viral RNA, removal of substances that inhibit nucleic acid amplification reactions, and the compatibility of sample treatment protocols with the subsequent nucleic acid amplification and detection techniques. The principles and approaches discussed in this review are applicable to molecular detection of other microbial pathogens. The Authors. Published by Elsevier B.V. 2023-08 2023-05-23 /pmc/articles/PMC10204347/ /pubmed/37317683 http://dx.doi.org/10.1016/j.trac.2023.117107 Text en © 2023 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Liu, Yanming
Kumblathan, Teresa
Tao, Jeffrey
Xu, Jingyang
Feng, Wei
Xiao, Huyan
Hu, Jianyu
Huang, Camille V.
Wu, Yiping
Zhang, Hongquan
Li, Xing-Fang
Le, X. Chris
Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title_full Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title_fullStr Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title_full_unstemmed Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title_short Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle
title_sort recent advances in rna sample preparation techniques for the detection of sars-cov-2 in saliva and gargle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204347/
https://www.ncbi.nlm.nih.gov/pubmed/37317683
http://dx.doi.org/10.1016/j.trac.2023.117107
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