A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis

The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST). In this study, we improved the cohesive and sticky p...

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Detalles Bibliográficos
Autores principales: Emoto, Yuri, Katayama, Ryoya, Hibino, Emi, Ishihara, Sho, Goda, Natsuko, Tenno, Takeshi, Kobashigawa, Yoshihiro, Morioka, Hiroshi, Hiroaki, Hidekazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204415/
https://www.ncbi.nlm.nih.gov/pubmed/37218904
http://dx.doi.org/10.3390/mps6030044

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