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Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test

Background: Since sensitivity and specificity vary widely between tests, SARS-CoV-2 serology results should be interpreted with caution. Methods: The study included serum samples from patients who had recovered from COVID-19 (n = 71), individuals vaccinated against SARS-CoV-2 (n = 84), and asymptoma...

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Autores principales: Vilibic-Cavlek, Tatjana, Bogdanic, Maja, Borko, Ema, Hruskar, Zeljka, Zilic, Denis, Ferenc, Thomas, Tabain, Irena, Barbic, Ljubo, Vujica Ferenc, Mateja, Ferencak, Ivana, Stevanovic, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204460/
https://www.ncbi.nlm.nih.gov/pubmed/37218901
http://dx.doi.org/10.3390/antib12020035
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author Vilibic-Cavlek, Tatjana
Bogdanic, Maja
Borko, Ema
Hruskar, Zeljka
Zilic, Denis
Ferenc, Thomas
Tabain, Irena
Barbic, Ljubo
Vujica Ferenc, Mateja
Ferencak, Ivana
Stevanovic, Vladimir
author_facet Vilibic-Cavlek, Tatjana
Bogdanic, Maja
Borko, Ema
Hruskar, Zeljka
Zilic, Denis
Ferenc, Thomas
Tabain, Irena
Barbic, Ljubo
Vujica Ferenc, Mateja
Ferencak, Ivana
Stevanovic, Vladimir
author_sort Vilibic-Cavlek, Tatjana
collection PubMed
description Background: Since sensitivity and specificity vary widely between tests, SARS-CoV-2 serology results should be interpreted with caution. Methods: The study included serum samples from patients who had recovered from COVID-19 (n = 71), individuals vaccinated against SARS-CoV-2 (n = 84), and asymptomatic individuals (n = 33). All samples were tested for the presence of binding antibodies (enzyme immunoassay; EIA), neutralizing (NT) antibodies (virus neutralization test; VNT), and surrogate NT (sNT) antibodies (surrogate virus neutralization test; sVNT) of SARS-CoV-2. Results: SARS-CoV-2-binding antibodies were detected in 71 (100%) COVID-19 patients, 77 (91.6%) vaccinated individuals, and 4 (12.1%) control subjects. Among EIA-positive samples, VNT was positive (titer ≥ 8) in 100% of COVID-19 patients and 63 (75.0%) of the vaccinated individuals, while sVNT was positive (>30% inhibition) in 62 (87.3%) patients and 59 (70.2%) vaccinated individuals. The analysis of antibody levels showed a significant moderate positive correlation between EIA and VNT, a moderate positive correlation between EIA and sVNT, and a strong positive correlation between VNT and sVNT. The proportion of positive sVNT detection rate was associated with VNT titer. The lowest positivity (72.4%/70.8%) was detected in samples with low NT titers (8/16) and increased progressively from 88.2% in samples with titer 32 to 100% in samples with titer 256. Conclusions: sVNT appeared to be a reliable method for the assessment COVID-19 serology in patients with high antibody levels, while false-negative results were frequently observed in patients with low NT titers.
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spelling pubmed-102044602023-05-24 Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test Vilibic-Cavlek, Tatjana Bogdanic, Maja Borko, Ema Hruskar, Zeljka Zilic, Denis Ferenc, Thomas Tabain, Irena Barbic, Ljubo Vujica Ferenc, Mateja Ferencak, Ivana Stevanovic, Vladimir Antibodies (Basel) Communication Background: Since sensitivity and specificity vary widely between tests, SARS-CoV-2 serology results should be interpreted with caution. Methods: The study included serum samples from patients who had recovered from COVID-19 (n = 71), individuals vaccinated against SARS-CoV-2 (n = 84), and asymptomatic individuals (n = 33). All samples were tested for the presence of binding antibodies (enzyme immunoassay; EIA), neutralizing (NT) antibodies (virus neutralization test; VNT), and surrogate NT (sNT) antibodies (surrogate virus neutralization test; sVNT) of SARS-CoV-2. Results: SARS-CoV-2-binding antibodies were detected in 71 (100%) COVID-19 patients, 77 (91.6%) vaccinated individuals, and 4 (12.1%) control subjects. Among EIA-positive samples, VNT was positive (titer ≥ 8) in 100% of COVID-19 patients and 63 (75.0%) of the vaccinated individuals, while sVNT was positive (>30% inhibition) in 62 (87.3%) patients and 59 (70.2%) vaccinated individuals. The analysis of antibody levels showed a significant moderate positive correlation between EIA and VNT, a moderate positive correlation between EIA and sVNT, and a strong positive correlation between VNT and sVNT. The proportion of positive sVNT detection rate was associated with VNT titer. The lowest positivity (72.4%/70.8%) was detected in samples with low NT titers (8/16) and increased progressively from 88.2% in samples with titer 32 to 100% in samples with titer 256. Conclusions: sVNT appeared to be a reliable method for the assessment COVID-19 serology in patients with high antibody levels, while false-negative results were frequently observed in patients with low NT titers. MDPI 2023-05-10 /pmc/articles/PMC10204460/ /pubmed/37218901 http://dx.doi.org/10.3390/antib12020035 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Vilibic-Cavlek, Tatjana
Bogdanic, Maja
Borko, Ema
Hruskar, Zeljka
Zilic, Denis
Ferenc, Thomas
Tabain, Irena
Barbic, Ljubo
Vujica Ferenc, Mateja
Ferencak, Ivana
Stevanovic, Vladimir
Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title_full Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title_fullStr Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title_full_unstemmed Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title_short Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
title_sort detection of sars-cov-2 antibodies: comparison of enzyme immunoassay, surrogate neutralization and virus neutralization test
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204460/
https://www.ncbi.nlm.nih.gov/pubmed/37218901
http://dx.doi.org/10.3390/antib12020035
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