Cargando…

Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry

Protein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically com...

Descripción completa

Detalles Bibliográficos
Autores principales: De Figueiredo, Isabel, Bartenlian, Bernard, Van der Rest, Guillaume, Pallandre, Antoine, Halgand, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204491/
https://www.ncbi.nlm.nih.gov/pubmed/37218924
http://dx.doi.org/10.3390/proteomes11020019
_version_ 1785045846554836992
author De Figueiredo, Isabel
Bartenlian, Bernard
Van der Rest, Guillaume
Pallandre, Antoine
Halgand, Frédéric
author_facet De Figueiredo, Isabel
Bartenlian, Bernard
Van der Rest, Guillaume
Pallandre, Antoine
Halgand, Frédéric
author_sort De Figueiredo, Isabel
collection PubMed
description Protein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically complex proteome (e.g., blood) making their detection difficult. This complexity is further increased by the needs to detect proteoforms and proteome complexity such as the dynamic range of compound concentrations. The development of techniques that simultaneously pre-concentrate and identify low-abundance biomarkers in these proteomes constitutes an avant-garde approach to the early detection of pathologies. Chromatographic-based methods are widely used for protein separation, but these methods are not adapted for biomarker discovery, as they require complex sample handling due to the low biomarker concentration. Therefore, microfluidics devices have emerged as a technology to overcome these shortcomings. In terms of detection, mass spectrometry (MS) is the standard analytical tool given its high sensitivity and specificity. However, for MS, the biomarker must be introduced as pure as possible in order to avoid chemical noise and improve sensitivity. As a result, microfluidics coupled with MS has become increasingly popular in the field of biomarker discovery. This review will show the different approaches to protein enrichment using miniaturized devices and the importance of their coupling with MS.
format Online
Article
Text
id pubmed-10204491
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102044912023-05-24 Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry De Figueiredo, Isabel Bartenlian, Bernard Van der Rest, Guillaume Pallandre, Antoine Halgand, Frédéric Proteomes Review Protein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically complex proteome (e.g., blood) making their detection difficult. This complexity is further increased by the needs to detect proteoforms and proteome complexity such as the dynamic range of compound concentrations. The development of techniques that simultaneously pre-concentrate and identify low-abundance biomarkers in these proteomes constitutes an avant-garde approach to the early detection of pathologies. Chromatographic-based methods are widely used for protein separation, but these methods are not adapted for biomarker discovery, as they require complex sample handling due to the low biomarker concentration. Therefore, microfluidics devices have emerged as a technology to overcome these shortcomings. In terms of detection, mass spectrometry (MS) is the standard analytical tool given its high sensitivity and specificity. However, for MS, the biomarker must be introduced as pure as possible in order to avoid chemical noise and improve sensitivity. As a result, microfluidics coupled with MS has become increasingly popular in the field of biomarker discovery. This review will show the different approaches to protein enrichment using miniaturized devices and the importance of their coupling with MS. MDPI 2023-05-10 /pmc/articles/PMC10204491/ /pubmed/37218924 http://dx.doi.org/10.3390/proteomes11020019 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
De Figueiredo, Isabel
Bartenlian, Bernard
Van der Rest, Guillaume
Pallandre, Antoine
Halgand, Frédéric
Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title_full Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title_fullStr Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title_full_unstemmed Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title_short Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
title_sort proteomics methodologies: the search of protein biomarkers using microfluidic systems coupled to mass spectrometry
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204491/
https://www.ncbi.nlm.nih.gov/pubmed/37218924
http://dx.doi.org/10.3390/proteomes11020019
work_keys_str_mv AT defigueiredoisabel proteomicsmethodologiesthesearchofproteinbiomarkersusingmicrofluidicsystemscoupledtomassspectrometry
AT bartenlianbernard proteomicsmethodologiesthesearchofproteinbiomarkersusingmicrofluidicsystemscoupledtomassspectrometry
AT vanderrestguillaume proteomicsmethodologiesthesearchofproteinbiomarkersusingmicrofluidicsystemscoupledtomassspectrometry
AT pallandreantoine proteomicsmethodologiesthesearchofproteinbiomarkersusingmicrofluidicsystemscoupledtomassspectrometry
AT halgandfrederic proteomicsmethodologiesthesearchofproteinbiomarkersusingmicrofluidicsystemscoupledtomassspectrometry