Cargando…

T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes

Type II diabetes (T2D) is a growing health problem worldwide due to increased levels of obesity and can lead to other life-threatening diseases, such as cardiovascular and kidney diseases. As the number of individuals diagnosed with T2D rises, there is an urgent need to understand the pathogenesis o...

Descripción completa

Detalles Bibliográficos
Autores principales: Distefano, Rebecca, Ilieva, Mirolyuba, Madsen, Jens Hedelund, Ishii, Hideshi, Aikawa, Masanori, Rennie, Sarah, Uchida, Shizuka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204529/
https://www.ncbi.nlm.nih.gov/pubmed/37218990
http://dx.doi.org/10.3390/ncrna9030030
_version_ 1785045855591464960
author Distefano, Rebecca
Ilieva, Mirolyuba
Madsen, Jens Hedelund
Ishii, Hideshi
Aikawa, Masanori
Rennie, Sarah
Uchida, Shizuka
author_facet Distefano, Rebecca
Ilieva, Mirolyuba
Madsen, Jens Hedelund
Ishii, Hideshi
Aikawa, Masanori
Rennie, Sarah
Uchida, Shizuka
author_sort Distefano, Rebecca
collection PubMed
description Type II diabetes (T2D) is a growing health problem worldwide due to increased levels of obesity and can lead to other life-threatening diseases, such as cardiovascular and kidney diseases. As the number of individuals diagnosed with T2D rises, there is an urgent need to understand the pathogenesis of the disease in order to prevent further harm to the body caused by elevated blood glucose levels. Recent advances in long non-coding RNA (lncRNA) research may provide insights into the pathogenesis of T2D. Although lncRNAs can be readily detected in RNA sequencing (RNA-seq) data, most published datasets of T2D patients compared to healthy donors focus only on protein-coding genes, leaving lncRNAs to be undiscovered and understudied. To address this knowledge gap, we performed a secondary analysis of published RNA-seq data of T2D patients and of patients with related health complications to systematically analyze the expression changes of lncRNA genes in relation to the protein-coding genes. Since immune cells play important roles in T2D, we conducted loss-of-function experiments to provide functional data on the T2D-related lncRNA USP30-AS1, using an in vitro model of pro-inflammatory macrophage activation. To facilitate lncRNA research in T2D, we developed a web application, T2DB, to provide a one-stop-shop for expression profiling of protein-coding and lncRNA genes in T2D patients compared to healthy donors or subjects without T2D.
format Online
Article
Text
id pubmed-10204529
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102045292023-05-24 T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes Distefano, Rebecca Ilieva, Mirolyuba Madsen, Jens Hedelund Ishii, Hideshi Aikawa, Masanori Rennie, Sarah Uchida, Shizuka Noncoding RNA Article Type II diabetes (T2D) is a growing health problem worldwide due to increased levels of obesity and can lead to other life-threatening diseases, such as cardiovascular and kidney diseases. As the number of individuals diagnosed with T2D rises, there is an urgent need to understand the pathogenesis of the disease in order to prevent further harm to the body caused by elevated blood glucose levels. Recent advances in long non-coding RNA (lncRNA) research may provide insights into the pathogenesis of T2D. Although lncRNAs can be readily detected in RNA sequencing (RNA-seq) data, most published datasets of T2D patients compared to healthy donors focus only on protein-coding genes, leaving lncRNAs to be undiscovered and understudied. To address this knowledge gap, we performed a secondary analysis of published RNA-seq data of T2D patients and of patients with related health complications to systematically analyze the expression changes of lncRNA genes in relation to the protein-coding genes. Since immune cells play important roles in T2D, we conducted loss-of-function experiments to provide functional data on the T2D-related lncRNA USP30-AS1, using an in vitro model of pro-inflammatory macrophage activation. To facilitate lncRNA research in T2D, we developed a web application, T2DB, to provide a one-stop-shop for expression profiling of protein-coding and lncRNA genes in T2D patients compared to healthy donors or subjects without T2D. MDPI 2023-05-08 /pmc/articles/PMC10204529/ /pubmed/37218990 http://dx.doi.org/10.3390/ncrna9030030 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Distefano, Rebecca
Ilieva, Mirolyuba
Madsen, Jens Hedelund
Ishii, Hideshi
Aikawa, Masanori
Rennie, Sarah
Uchida, Shizuka
T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title_full T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title_fullStr T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title_full_unstemmed T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title_short T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes
title_sort t2db: a web database for long non-coding rna genes in type ii diabetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204529/
https://www.ncbi.nlm.nih.gov/pubmed/37218990
http://dx.doi.org/10.3390/ncrna9030030
work_keys_str_mv AT distefanorebecca t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT ilievamirolyuba t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT madsenjenshedelund t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT ishiihideshi t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT aikawamasanori t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT renniesarah t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes
AT uchidashizuka t2dbawebdatabaseforlongnoncodingrnagenesintypeiidiabetes