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Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis
BACKGROUND: High bacterial burden in the lung microbiota predicts progression of idiopathic pulmonary fibrosis (IPF). Azithromycin (AZT) is a macrolide antibiotic known to alter the lung microbiota in several chronic pulmonary diseases, and observational studies have shown a positive effect of AZT o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Respiratory Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204823/ https://www.ncbi.nlm.nih.gov/pubmed/37228285 http://dx.doi.org/10.1183/23120541.00720-2022 |
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author | Gijs, Pieter-Jan Daccord, Cécile Bernasconi, Eric Brutsche, Martin Clarenbach, Christian F. Hostettler, Katrin Guler, Sabina A. Mercier, Louis Ubags, Niki Funke-Chambour, Manuela von Garnier, Christophe |
author_facet | Gijs, Pieter-Jan Daccord, Cécile Bernasconi, Eric Brutsche, Martin Clarenbach, Christian F. Hostettler, Katrin Guler, Sabina A. Mercier, Louis Ubags, Niki Funke-Chambour, Manuela von Garnier, Christophe |
author_sort | Gijs, Pieter-Jan |
collection | PubMed |
description | BACKGROUND: High bacterial burden in the lung microbiota predicts progression of idiopathic pulmonary fibrosis (IPF). Azithromycin (AZT) is a macrolide antibiotic known to alter the lung microbiota in several chronic pulmonary diseases, and observational studies have shown a positive effect of AZT on mortality and hospitalisation rate in IPF. However, the effect of AZT on the lung microbiota in IPF remains unknown. METHODS: We sought to determine the impact of a 3-month course of AZT on the lung microbiota in IPF. We assessed sputum and oropharyngeal swab specimens from 24 adults with IPF included in a randomised controlled crossover trial of oral AZT 500 mg 3 times per week. 16S rRNA gene amplicon sequencing and quantitative PCR (qPCR) were performed to assess bacterial communities. Antibiotic resistance genes (ARGs) were assessed using real-time qPCR. RESULTS: AZT significantly decreased community diversity with a stronger and more persistent effect in the lower airways (sputum). AZT treatment altered the temporal kinetics of the upper (oropharyngeal swab) and lower airway microbiota, increasing community similarity between the two sites for 1 month after macrolide cessation. Patients with an increase in ARG carriage had lower bacterial density and enrichment of the genus Streptococcus. In contrast, patients with more stable ARG carriage had higher bacterial density and enrichment in Prevotella. CONCLUSIONS: AZT caused sustained changes in the diversity and composition of the upper and lower airway microbiota in IPF, with effects on the temporal and spatial dynamics between the two sites. |
format | Online Article Text |
id | pubmed-10204823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-102048232023-05-24 Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis Gijs, Pieter-Jan Daccord, Cécile Bernasconi, Eric Brutsche, Martin Clarenbach, Christian F. Hostettler, Katrin Guler, Sabina A. Mercier, Louis Ubags, Niki Funke-Chambour, Manuela von Garnier, Christophe ERJ Open Res Original Research Articles BACKGROUND: High bacterial burden in the lung microbiota predicts progression of idiopathic pulmonary fibrosis (IPF). Azithromycin (AZT) is a macrolide antibiotic known to alter the lung microbiota in several chronic pulmonary diseases, and observational studies have shown a positive effect of AZT on mortality and hospitalisation rate in IPF. However, the effect of AZT on the lung microbiota in IPF remains unknown. METHODS: We sought to determine the impact of a 3-month course of AZT on the lung microbiota in IPF. We assessed sputum and oropharyngeal swab specimens from 24 adults with IPF included in a randomised controlled crossover trial of oral AZT 500 mg 3 times per week. 16S rRNA gene amplicon sequencing and quantitative PCR (qPCR) were performed to assess bacterial communities. Antibiotic resistance genes (ARGs) were assessed using real-time qPCR. RESULTS: AZT significantly decreased community diversity with a stronger and more persistent effect in the lower airways (sputum). AZT treatment altered the temporal kinetics of the upper (oropharyngeal swab) and lower airway microbiota, increasing community similarity between the two sites for 1 month after macrolide cessation. Patients with an increase in ARG carriage had lower bacterial density and enrichment of the genus Streptococcus. In contrast, patients with more stable ARG carriage had higher bacterial density and enrichment in Prevotella. CONCLUSIONS: AZT caused sustained changes in the diversity and composition of the upper and lower airway microbiota in IPF, with effects on the temporal and spatial dynamics between the two sites. European Respiratory Society 2023-05-22 /pmc/articles/PMC10204823/ /pubmed/37228285 http://dx.doi.org/10.1183/23120541.00720-2022 Text en Copyright ©The authors 2023 https://creativecommons.org/licenses/by/4.0/This version is distributed under the terms of the Creative Commons Attribution Licence 4.0. |
spellingShingle | Original Research Articles Gijs, Pieter-Jan Daccord, Cécile Bernasconi, Eric Brutsche, Martin Clarenbach, Christian F. Hostettler, Katrin Guler, Sabina A. Mercier, Louis Ubags, Niki Funke-Chambour, Manuela von Garnier, Christophe Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title | Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title_full | Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title_fullStr | Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title_full_unstemmed | Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title_short | Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
title_sort | azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis |
topic | Original Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204823/ https://www.ncbi.nlm.nih.gov/pubmed/37228285 http://dx.doi.org/10.1183/23120541.00720-2022 |
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