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Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt
The similarity of the Listeria innocua genome with Listeria monocytogenes and their presence in the same niche may facilitate gene transfer between them. A better understanding of the mechanisms responsible for bacterial virulence requires an in-depth knowledge of the genetic characteristics of thes...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206011/ https://www.ncbi.nlm.nih.gov/pubmed/37234542 http://dx.doi.org/10.3389/fmicb.2023.1160244 |
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author | Ramadan, Hazem Al-Ashmawy, Maha Soliman, Ahmed M. Elbediwi, Mohammed Sabeq, Islam Yousef, Mona Algammal, Abdelazeem M. Hiott, Lari M. Berrang, Mark E. Frye, Jonathan G. Jackson, Charlene R. |
author_facet | Ramadan, Hazem Al-Ashmawy, Maha Soliman, Ahmed M. Elbediwi, Mohammed Sabeq, Islam Yousef, Mona Algammal, Abdelazeem M. Hiott, Lari M. Berrang, Mark E. Frye, Jonathan G. Jackson, Charlene R. |
author_sort | Ramadan, Hazem |
collection | PubMed |
description | The similarity of the Listeria innocua genome with Listeria monocytogenes and their presence in the same niche may facilitate gene transfer between them. A better understanding of the mechanisms responsible for bacterial virulence requires an in-depth knowledge of the genetic characteristics of these bacteria. In this context, draft whole genome sequences were completed on five L. innocua isolated from milk and dairy products in Egypt. The assembled sequences were screened for antimicrobial resistance and virulence genes, plasmid replicons and multilocus sequence types (MLST); phylogenetic analysis of the sequenced isolates was also performed. The sequencing results revealed the presence of only one antimicrobial resistance gene, fosX, in the L. innocua isolates. However, the five isolates carried 13 virulence genes involved in adhesion, invasion, surface protein anchoring, peptidoglycan degradation, intracellular survival, and heat stress; all five lacked the Listeria Pathogenicity Island 1 (LIPI-1) genes. MLST assigned these five isolates into the same sequence type (ST), ST-1085; however, single nucleotide polymorphism (SNP)-based phylogenetic analysis revealed 422–1,091 SNP differences between our isolates and global lineages of L. innocua. The five isolates possessed an ATP-dependent protease (clpL) gene, which mediates heat resistance, on a rep25 type plasmids. Blast analysis of clpL-carrying plasmid contigs showed approximately 99% sequence similarity to the corresponding parts of plasmids of L. monocytogenes strains 2015TE24968 and N1-011A previously isolated from Italy and the United States, respectively. Although this plasmid has been linked to L. monocytogenes that was responsible for a serious outbreak, this is the first report of L. innocua containing clpL-carrying plasmids. Various genetic mechanisms of virulence transfer among Listeria species and other genera could raise the possibility of the evolution of virulent strains of L. innocua. Such strains could challenge processing and preservation protocols and pose health risks from dairy products. Ongoing genomic research is necessary to identify these alarming genetic changes and develop preventive and control measures. |
format | Online Article Text |
id | pubmed-10206011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102060112023-05-25 Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt Ramadan, Hazem Al-Ashmawy, Maha Soliman, Ahmed M. Elbediwi, Mohammed Sabeq, Islam Yousef, Mona Algammal, Abdelazeem M. Hiott, Lari M. Berrang, Mark E. Frye, Jonathan G. Jackson, Charlene R. Front Microbiol Microbiology The similarity of the Listeria innocua genome with Listeria monocytogenes and their presence in the same niche may facilitate gene transfer between them. A better understanding of the mechanisms responsible for bacterial virulence requires an in-depth knowledge of the genetic characteristics of these bacteria. In this context, draft whole genome sequences were completed on five L. innocua isolated from milk and dairy products in Egypt. The assembled sequences were screened for antimicrobial resistance and virulence genes, plasmid replicons and multilocus sequence types (MLST); phylogenetic analysis of the sequenced isolates was also performed. The sequencing results revealed the presence of only one antimicrobial resistance gene, fosX, in the L. innocua isolates. However, the five isolates carried 13 virulence genes involved in adhesion, invasion, surface protein anchoring, peptidoglycan degradation, intracellular survival, and heat stress; all five lacked the Listeria Pathogenicity Island 1 (LIPI-1) genes. MLST assigned these five isolates into the same sequence type (ST), ST-1085; however, single nucleotide polymorphism (SNP)-based phylogenetic analysis revealed 422–1,091 SNP differences between our isolates and global lineages of L. innocua. The five isolates possessed an ATP-dependent protease (clpL) gene, which mediates heat resistance, on a rep25 type plasmids. Blast analysis of clpL-carrying plasmid contigs showed approximately 99% sequence similarity to the corresponding parts of plasmids of L. monocytogenes strains 2015TE24968 and N1-011A previously isolated from Italy and the United States, respectively. Although this plasmid has been linked to L. monocytogenes that was responsible for a serious outbreak, this is the first report of L. innocua containing clpL-carrying plasmids. Various genetic mechanisms of virulence transfer among Listeria species and other genera could raise the possibility of the evolution of virulent strains of L. innocua. Such strains could challenge processing and preservation protocols and pose health risks from dairy products. Ongoing genomic research is necessary to identify these alarming genetic changes and develop preventive and control measures. Frontiers Media S.A. 2023-05-10 /pmc/articles/PMC10206011/ /pubmed/37234542 http://dx.doi.org/10.3389/fmicb.2023.1160244 Text en Copyright © 2023 Ramadan, Al-Ashmawy, Soliman, Elbediwi, Sabeq, Yousef, Algammal, Hiott, Berrang, Frye and Jackson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ramadan, Hazem Al-Ashmawy, Maha Soliman, Ahmed M. Elbediwi, Mohammed Sabeq, Islam Yousef, Mona Algammal, Abdelazeem M. Hiott, Lari M. Berrang, Mark E. Frye, Jonathan G. Jackson, Charlene R. Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title | Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title_full | Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title_fullStr | Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title_full_unstemmed | Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title_short | Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt |
title_sort | whole-genome sequencing of listeria innocua recovered from retail milk and dairy products in egypt |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206011/ https://www.ncbi.nlm.nih.gov/pubmed/37234542 http://dx.doi.org/10.3389/fmicb.2023.1160244 |
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