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The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization

This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, an...

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Autor principal: Morgan, Kalindi D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206073/
https://www.ncbi.nlm.nih.gov/pubmed/37234518
http://dx.doi.org/10.3389/fmicb.2023.1174591
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author Morgan, Kalindi D.
author_facet Morgan, Kalindi D.
author_sort Morgan, Kalindi D.
collection PubMed
description This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, and hybrid natural products. Nitrogen-15 can be detected at natural abundance utilizing two-dimensional nuclear magnetic resonance and mass spectrometry. Additionally, it is a stable isotope that can be added to growth media for both filamentous fungi and bacteria. With stable isotope feeding, additional two-dimensional nuclear magnetic resonance and mass spectrometry strategies have become available, and there is a growing trend to use nitrogen-15 stable isotope feeding for the biosynthetic characterization of natural products. This mini-review will catalog the use of these strategies, analyze the strengths and weaknesses of the different approaches, and suggest future directions for the use of nitrogen-15 in natural product discovery and biosynthetic characterization.
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spelling pubmed-102060732023-05-25 The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization Morgan, Kalindi D. Front Microbiol Microbiology This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, and hybrid natural products. Nitrogen-15 can be detected at natural abundance utilizing two-dimensional nuclear magnetic resonance and mass spectrometry. Additionally, it is a stable isotope that can be added to growth media for both filamentous fungi and bacteria. With stable isotope feeding, additional two-dimensional nuclear magnetic resonance and mass spectrometry strategies have become available, and there is a growing trend to use nitrogen-15 stable isotope feeding for the biosynthetic characterization of natural products. This mini-review will catalog the use of these strategies, analyze the strengths and weaknesses of the different approaches, and suggest future directions for the use of nitrogen-15 in natural product discovery and biosynthetic characterization. Frontiers Media S.A. 2023-05-10 /pmc/articles/PMC10206073/ /pubmed/37234518 http://dx.doi.org/10.3389/fmicb.2023.1174591 Text en Copyright © 2023 Morgan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Morgan, Kalindi D.
The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title_full The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title_fullStr The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title_full_unstemmed The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title_short The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
title_sort use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206073/
https://www.ncbi.nlm.nih.gov/pubmed/37234518
http://dx.doi.org/10.3389/fmicb.2023.1174591
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