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The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization
This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, an...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206073/ https://www.ncbi.nlm.nih.gov/pubmed/37234518 http://dx.doi.org/10.3389/fmicb.2023.1174591 |
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author | Morgan, Kalindi D. |
author_facet | Morgan, Kalindi D. |
author_sort | Morgan, Kalindi D. |
collection | PubMed |
description | This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, and hybrid natural products. Nitrogen-15 can be detected at natural abundance utilizing two-dimensional nuclear magnetic resonance and mass spectrometry. Additionally, it is a stable isotope that can be added to growth media for both filamentous fungi and bacteria. With stable isotope feeding, additional two-dimensional nuclear magnetic resonance and mass spectrometry strategies have become available, and there is a growing trend to use nitrogen-15 stable isotope feeding for the biosynthetic characterization of natural products. This mini-review will catalog the use of these strategies, analyze the strengths and weaknesses of the different approaches, and suggest future directions for the use of nitrogen-15 in natural product discovery and biosynthetic characterization. |
format | Online Article Text |
id | pubmed-10206073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102060732023-05-25 The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization Morgan, Kalindi D. Front Microbiol Microbiology This mini-review covers the use of nitrogen-15 in bacterial and fungal natural product discovery and biosynthetic characterization from 1970 to 2022. Nitrogen is an important element in a number of bioactive and structurally intriguing natural products including alkaloids, non-ribosomal peptides, and hybrid natural products. Nitrogen-15 can be detected at natural abundance utilizing two-dimensional nuclear magnetic resonance and mass spectrometry. Additionally, it is a stable isotope that can be added to growth media for both filamentous fungi and bacteria. With stable isotope feeding, additional two-dimensional nuclear magnetic resonance and mass spectrometry strategies have become available, and there is a growing trend to use nitrogen-15 stable isotope feeding for the biosynthetic characterization of natural products. This mini-review will catalog the use of these strategies, analyze the strengths and weaknesses of the different approaches, and suggest future directions for the use of nitrogen-15 in natural product discovery and biosynthetic characterization. Frontiers Media S.A. 2023-05-10 /pmc/articles/PMC10206073/ /pubmed/37234518 http://dx.doi.org/10.3389/fmicb.2023.1174591 Text en Copyright © 2023 Morgan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Morgan, Kalindi D. The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title | The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title_full | The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title_fullStr | The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title_full_unstemmed | The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title_short | The use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
title_sort | use of nitrogen-15 in microbial natural product discovery and biosynthetic characterization |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206073/ https://www.ncbi.nlm.nih.gov/pubmed/37234518 http://dx.doi.org/10.3389/fmicb.2023.1174591 |
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