Protocol for IPO11 deletion and re-expression in H460 lung cancer cells using CRISPR-Cas9 and plasmid transfection

Exploring the essential role of Importin 11 (IPO11) in the nuclear translocation of its potential cargo proteins requires an efficient means of IPO11 deletion and re-expression. Here, we present a protocol for the generation of IPO11 deletion using CRISPR-Cas9 and re-expression using plasmid transfe...

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Detalles Bibliográficos
Autores principales: Zhang, Yarui, Ou, Guomin, Zhou, Zhou, Wang, Jingdong, Yang, Hongying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206203/
https://www.ncbi.nlm.nih.gov/pubmed/37195868
http://dx.doi.org/10.1016/j.xpro.2023.102317

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