A simple smiFISH pipeline to quantify mRNA at the single-cell level in 3D

Techniques allowing the precise quantification of mRNA at the cellular level are essential for understanding biological processes. Here, we present a semi-automated smiFISH (single-molecule inexpensive FISH) pipeline enabling quantification of mRNA in a small number of cells (∼40) in fixed whole mou...

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Detalles Bibliográficos
Autores principales: Bouchet, Mathilde, Urdy, Séverine, Guan, Wenyue, Kabir, Chérif, Garvis, Steve, Enriquez, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206206/
https://www.ncbi.nlm.nih.gov/pubmed/37195867
http://dx.doi.org/10.1016/j.xpro.2023.102316

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