Cargando…
Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206225/ https://www.ncbi.nlm.nih.gov/pubmed/37234544 http://dx.doi.org/10.3389/fmicb.2023.1141418 |
_version_ | 1785046181723766784 |
---|---|
author | Liu, Lijuan Dong, Zhiyou Ai, Shengquan Chen, Shanyu Dong, Mengyao Li, Qianlan Zhou, Ziyao Liu, Haifeng Zhong, Zhijun Ma, Xiaoping Hu, Yanchun Ren, Zhihua Fu, Hualin Shu, Gang Qiu, Xianmeng Peng, Guangneng |
author_facet | Liu, Lijuan Dong, Zhiyou Ai, Shengquan Chen, Shanyu Dong, Mengyao Li, Qianlan Zhou, Ziyao Liu, Haifeng Zhong, Zhijun Ma, Xiaoping Hu, Yanchun Ren, Zhihua Fu, Hualin Shu, Gang Qiu, Xianmeng Peng, Guangneng |
author_sort | Liu, Lijuan |
collection | PubMed |
description | INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs). METHOD: In this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates. RESULTS: Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, bla(CTX-M), bla(CTX-M-2), bla(OXA-1), bla(TEM), tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: bla(TEM), bla(CTX-M), and bla(CTX-M-2). This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs. DISCUSSION: Given the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health. |
format | Online Article Text |
id | pubmed-10206225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102062252023-05-25 Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs Liu, Lijuan Dong, Zhiyou Ai, Shengquan Chen, Shanyu Dong, Mengyao Li, Qianlan Zhou, Ziyao Liu, Haifeng Zhong, Zhijun Ma, Xiaoping Hu, Yanchun Ren, Zhihua Fu, Hualin Shu, Gang Qiu, Xianmeng Peng, Guangneng Front Microbiol Microbiology INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs). METHOD: In this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates. RESULTS: Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, bla(CTX-M), bla(CTX-M-2), bla(OXA-1), bla(TEM), tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: bla(TEM), bla(CTX-M), and bla(CTX-M-2). This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs. DISCUSSION: Given the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health. Frontiers Media S.A. 2023-05-10 /pmc/articles/PMC10206225/ /pubmed/37234544 http://dx.doi.org/10.3389/fmicb.2023.1141418 Text en Copyright © 2023 Liu, Dong, Ai, Chen, Dong, Li, Zhou, Liu, Zhong, Ma, Hu, Ren, Fu, Shu, Qiu and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Lijuan Dong, Zhiyou Ai, Shengquan Chen, Shanyu Dong, Mengyao Li, Qianlan Zhou, Ziyao Liu, Haifeng Zhong, Zhijun Ma, Xiaoping Hu, Yanchun Ren, Zhihua Fu, Hualin Shu, Gang Qiu, Xianmeng Peng, Guangneng Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title | Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title_full | Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title_fullStr | Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title_full_unstemmed | Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title_short | Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs |
title_sort | virulence-related factors and antimicrobial resistance in proteus mirabilis isolated from domestic and stray dogs |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206225/ https://www.ncbi.nlm.nih.gov/pubmed/37234544 http://dx.doi.org/10.3389/fmicb.2023.1141418 |
work_keys_str_mv | AT liulijuan virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT dongzhiyou virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT aishengquan virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT chenshanyu virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT dongmengyao virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT liqianlan virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT zhouziyao virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT liuhaifeng virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT zhongzhijun virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT maxiaoping virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT huyanchun virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT renzhihua virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT fuhualin virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT shugang virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT qiuxianmeng virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs AT pengguangneng virulencerelatedfactorsandantimicrobialresistanceinproteusmirabilisisolatedfromdomesticandstraydogs |