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Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public...

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Autores principales: Liu, Lijuan, Dong, Zhiyou, Ai, Shengquan, Chen, Shanyu, Dong, Mengyao, Li, Qianlan, Zhou, Ziyao, Liu, Haifeng, Zhong, Zhijun, Ma, Xiaoping, Hu, Yanchun, Ren, Zhihua, Fu, Hualin, Shu, Gang, Qiu, Xianmeng, Peng, Guangneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206225/
https://www.ncbi.nlm.nih.gov/pubmed/37234544
http://dx.doi.org/10.3389/fmicb.2023.1141418
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author Liu, Lijuan
Dong, Zhiyou
Ai, Shengquan
Chen, Shanyu
Dong, Mengyao
Li, Qianlan
Zhou, Ziyao
Liu, Haifeng
Zhong, Zhijun
Ma, Xiaoping
Hu, Yanchun
Ren, Zhihua
Fu, Hualin
Shu, Gang
Qiu, Xianmeng
Peng, Guangneng
author_facet Liu, Lijuan
Dong, Zhiyou
Ai, Shengquan
Chen, Shanyu
Dong, Mengyao
Li, Qianlan
Zhou, Ziyao
Liu, Haifeng
Zhong, Zhijun
Ma, Xiaoping
Hu, Yanchun
Ren, Zhihua
Fu, Hualin
Shu, Gang
Qiu, Xianmeng
Peng, Guangneng
author_sort Liu, Lijuan
collection PubMed
description INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs). METHOD: In this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates. RESULTS: Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, bla(CTX-M), bla(CTX-M-2), bla(OXA-1), bla(TEM), tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: bla(TEM), bla(CTX-M), and bla(CTX-M-2). This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs. DISCUSSION: Given the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health.
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spelling pubmed-102062252023-05-25 Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs Liu, Lijuan Dong, Zhiyou Ai, Shengquan Chen, Shanyu Dong, Mengyao Li, Qianlan Zhou, Ziyao Liu, Haifeng Zhong, Zhijun Ma, Xiaoping Hu, Yanchun Ren, Zhihua Fu, Hualin Shu, Gang Qiu, Xianmeng Peng, Guangneng Front Microbiol Microbiology INTRODUCTION: Proteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs). METHOD: In this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates. RESULTS: Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, bla(CTX-M), bla(CTX-M-2), bla(OXA-1), bla(TEM), tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: bla(TEM), bla(CTX-M), and bla(CTX-M-2). This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs. DISCUSSION: Given the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health. Frontiers Media S.A. 2023-05-10 /pmc/articles/PMC10206225/ /pubmed/37234544 http://dx.doi.org/10.3389/fmicb.2023.1141418 Text en Copyright © 2023 Liu, Dong, Ai, Chen, Dong, Li, Zhou, Liu, Zhong, Ma, Hu, Ren, Fu, Shu, Qiu and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Lijuan
Dong, Zhiyou
Ai, Shengquan
Chen, Shanyu
Dong, Mengyao
Li, Qianlan
Zhou, Ziyao
Liu, Haifeng
Zhong, Zhijun
Ma, Xiaoping
Hu, Yanchun
Ren, Zhihua
Fu, Hualin
Shu, Gang
Qiu, Xianmeng
Peng, Guangneng
Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title_full Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title_fullStr Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title_full_unstemmed Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title_short Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs
title_sort virulence-related factors and antimicrobial resistance in proteus mirabilis isolated from domestic and stray dogs
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10206225/
https://www.ncbi.nlm.nih.gov/pubmed/37234544
http://dx.doi.org/10.3389/fmicb.2023.1141418
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