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Förster Resonance Energy Transfer Assay for Investigating the Reactivity of Thioesters in Biochemistry and Native Chemical Ligation

[Image: see text] Thioesters are considered to be “energy-rich” functional groups that are susceptible to attack by thiolate and amine nucleophiles while remaining hydrolytically stable at neutral pH, which enables thioester chemistry to take place in an aqueous medium. Thus, the inherent reactivity...

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Detalles Bibliográficos
Autores principales: Gless, Bengt H., Schmied, Sabrina H., Bejder, Benjamin S., Olsen, Christian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207088/
https://www.ncbi.nlm.nih.gov/pubmed/37234128
http://dx.doi.org/10.1021/jacsau.3c00095

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