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Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 n...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Journal of the ASEAN Federation of Endocrine Societies
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207871/ https://www.ncbi.nlm.nih.gov/pubmed/37234927 http://dx.doi.org/10.15605/jafes.037.S7 |
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author | Benjamin, Kimberly Marquez, Cielo Mae Morta, Madeleine Reyes, Emmanuel Marc Aragones, Lemnuel Velarde, Michael |
author_facet | Benjamin, Kimberly Marquez, Cielo Mae Morta, Madeleine Reyes, Emmanuel Marc Aragones, Lemnuel Velarde, Michael |
author_sort | Benjamin, Kimberly |
collection | PubMed |
description | OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 nM and 100 nM) for 72 hours. Cell proliferation was assessed through the viability assays MTT and CellTiter-Glo(®). Wound healing assays were also used to evaluate the migration potential of the cell line. The expression of genes related to proliferation and migration was also determined. Similarly, adult mice were exposed to BPS at a dose of 30 mg/kg body weight/day for 21 days, after which, the uterus was sent for histopathologic assessment. RESULTS: BPS increased cell number and stimulated migration in Ishikawa cells, in association with the upregulation of estrogen receptor beta (ESR2) and vimentin (VIM). In addition, mice exposed to BPS showed a significantly higher mean number of endometrial glands within the endometrium. CONCLUSION: Overall, in vitro and in vivo results obtained in this study showed that BPS could significantly promote endometrial epithelial cell proliferation and migration, a phenotype also observed with BPA exposure. Hence, the use of BPS in BPA-free products must be reassessed, as it may pose adverse reproductive health effects to humans. |
format | Online Article Text |
id | pubmed-10207871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Journal of the ASEAN Federation of Endocrine Societies |
record_format | MEDLINE/PubMed |
spelling | pubmed-102078712023-05-25 Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells Benjamin, Kimberly Marquez, Cielo Mae Morta, Madeleine Reyes, Emmanuel Marc Aragones, Lemnuel Velarde, Michael J ASEAN Fed Endocr Soc Original Article OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 nM and 100 nM) for 72 hours. Cell proliferation was assessed through the viability assays MTT and CellTiter-Glo(®). Wound healing assays were also used to evaluate the migration potential of the cell line. The expression of genes related to proliferation and migration was also determined. Similarly, adult mice were exposed to BPS at a dose of 30 mg/kg body weight/day for 21 days, after which, the uterus was sent for histopathologic assessment. RESULTS: BPS increased cell number and stimulated migration in Ishikawa cells, in association with the upregulation of estrogen receptor beta (ESR2) and vimentin (VIM). In addition, mice exposed to BPS showed a significantly higher mean number of endometrial glands within the endometrium. CONCLUSION: Overall, in vitro and in vivo results obtained in this study showed that BPS could significantly promote endometrial epithelial cell proliferation and migration, a phenotype also observed with BPA exposure. Hence, the use of BPS in BPA-free products must be reassessed, as it may pose adverse reproductive health effects to humans. Journal of the ASEAN Federation of Endocrine Societies 2022-08-06 2023 /pmc/articles/PMC10207871/ /pubmed/37234927 http://dx.doi.org/10.15605/jafes.037.S7 Text en © 2022 Journal of the ASEAN Federation of Endocrine Societies https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
spellingShingle | Original Article Benjamin, Kimberly Marquez, Cielo Mae Morta, Madeleine Reyes, Emmanuel Marc Aragones, Lemnuel Velarde, Michael Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title | Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title_full | Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title_fullStr | Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title_full_unstemmed | Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title_short | Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells |
title_sort | bisphenol s increases cell number and stimulates migration of endometrial epithelial cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207871/ https://www.ncbi.nlm.nih.gov/pubmed/37234927 http://dx.doi.org/10.15605/jafes.037.S7 |
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