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Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells

OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 n...

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Autores principales: Benjamin, Kimberly, Marquez, Cielo Mae, Morta, Madeleine, Reyes, Emmanuel Marc, Aragones, Lemnuel, Velarde, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of the ASEAN Federation of Endocrine Societies 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207871/
https://www.ncbi.nlm.nih.gov/pubmed/37234927
http://dx.doi.org/10.15605/jafes.037.S7
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author Benjamin, Kimberly
Marquez, Cielo Mae
Morta, Madeleine
Reyes, Emmanuel Marc
Aragones, Lemnuel
Velarde, Michael
author_facet Benjamin, Kimberly
Marquez, Cielo Mae
Morta, Madeleine
Reyes, Emmanuel Marc
Aragones, Lemnuel
Velarde, Michael
author_sort Benjamin, Kimberly
collection PubMed
description OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 nM and 100 nM) for 72 hours. Cell proliferation was assessed through the viability assays MTT and CellTiter-Glo(®). Wound healing assays were also used to evaluate the migration potential of the cell line. The expression of genes related to proliferation and migration was also determined. Similarly, adult mice were exposed to BPS at a dose of 30 mg/kg body weight/day for 21 days, after which, the uterus was sent for histopathologic assessment. RESULTS: BPS increased cell number and stimulated migration in Ishikawa cells, in association with the upregulation of estrogen receptor beta (ESR2) and vimentin (VIM). In addition, mice exposed to BPS showed a significantly higher mean number of endometrial glands within the endometrium. CONCLUSION: Overall, in vitro and in vivo results obtained in this study showed that BPS could significantly promote endometrial epithelial cell proliferation and migration, a phenotype also observed with BPA exposure. Hence, the use of BPS in BPA-free products must be reassessed, as it may pose adverse reproductive health effects to humans.
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spelling pubmed-102078712023-05-25 Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells Benjamin, Kimberly Marquez, Cielo Mae Morta, Madeleine Reyes, Emmanuel Marc Aragones, Lemnuel Velarde, Michael J ASEAN Fed Endocr Soc Original Article OBJECTIVE: To determine whether bisphenol S (BPS), a common substitute for bisphenol A (BPA), induces cell proliferation and migration in human endometrial epithelial cells (Ishikawa) and adult mouse uterine tissues. METHODOLOGY: Human endometrial Ishikawa cells were exposed to low doses of BPS (1 nM and 100 nM) for 72 hours. Cell proliferation was assessed through the viability assays MTT and CellTiter-Glo(®). Wound healing assays were also used to evaluate the migration potential of the cell line. The expression of genes related to proliferation and migration was also determined. Similarly, adult mice were exposed to BPS at a dose of 30 mg/kg body weight/day for 21 days, after which, the uterus was sent for histopathologic assessment. RESULTS: BPS increased cell number and stimulated migration in Ishikawa cells, in association with the upregulation of estrogen receptor beta (ESR2) and vimentin (VIM). In addition, mice exposed to BPS showed a significantly higher mean number of endometrial glands within the endometrium. CONCLUSION: Overall, in vitro and in vivo results obtained in this study showed that BPS could significantly promote endometrial epithelial cell proliferation and migration, a phenotype also observed with BPA exposure. Hence, the use of BPS in BPA-free products must be reassessed, as it may pose adverse reproductive health effects to humans. Journal of the ASEAN Federation of Endocrine Societies 2022-08-06 2023 /pmc/articles/PMC10207871/ /pubmed/37234927 http://dx.doi.org/10.15605/jafes.037.S7 Text en © 2022 Journal of the ASEAN Federation of Endocrine Societies https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
spellingShingle Original Article
Benjamin, Kimberly
Marquez, Cielo Mae
Morta, Madeleine
Reyes, Emmanuel Marc
Aragones, Lemnuel
Velarde, Michael
Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title_full Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title_fullStr Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title_full_unstemmed Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title_short Bisphenol S Increases Cell Number and Stimulates Migration of Endometrial Epithelial Cells
title_sort bisphenol s increases cell number and stimulates migration of endometrial epithelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207871/
https://www.ncbi.nlm.nih.gov/pubmed/37234927
http://dx.doi.org/10.15605/jafes.037.S7
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