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A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs

COVID-19 has afflicted people's lives worldwide. Interleukin-6 (IL-6) is an important COVID-19 biomarker in human body fluids that can be used as a reference to monitor COVID-19 in real-time and therefore to reduce the risk of virus transmission. On the other hand, oseltamivir is a potential CO...

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Autores principales: Wang, Xinrui, Clavier, Gilles, Zhang, Yan, Batra, Kamal, Xiao, Nanan, Maurin, Guillaume, Ding, Bin, Tissot, Antoine, Serre, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207894/
https://www.ncbi.nlm.nih.gov/pubmed/37234896
http://dx.doi.org/10.1039/d3sc00106g
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author Wang, Xinrui
Clavier, Gilles
Zhang, Yan
Batra, Kamal
Xiao, Nanan
Maurin, Guillaume
Ding, Bin
Tissot, Antoine
Serre, Christian
author_facet Wang, Xinrui
Clavier, Gilles
Zhang, Yan
Batra, Kamal
Xiao, Nanan
Maurin, Guillaume
Ding, Bin
Tissot, Antoine
Serre, Christian
author_sort Wang, Xinrui
collection PubMed
description COVID-19 has afflicted people's lives worldwide. Interleukin-6 (IL-6) is an important COVID-19 biomarker in human body fluids that can be used as a reference to monitor COVID-19 in real-time and therefore to reduce the risk of virus transmission. On the other hand, oseltamivir is a potential COVID-19 curing drug, but its overuse easily leads to hazardous side effects, calling for its real time monitoring in body fluids. For these purposes, a new yttrium metal–organic framework (Y-MOF) has been synthesized, in which the 5-(4-(imidazole-1-yl)phenyl)isophthalic linker contains a large aromatic backbone capable of strongly interacting with DNA sequences through π–π stacking interactions, which makes it appealing to build a unique sensor based on DNA functionalized MOFs. The MOF/DNA sequence hybrid luminescent sensing platform presents excellent optical properties associated with a high Förster resonance energy transfer (FRET) efficiency. Furthermore, to construct a dual emission sensing platform, a 5′-carboxylfluorescein (FAM) labeled DNA sequence (S2) with a stem-loop structure that can specifically interact with IL-6 has been associated with the Y-MOF. The resulting Y-MOF@S2 exhibits an efficient ratiometric detection of IL-6 in human body fluids with an extremely high K(sv) value 4.3 × 10(8) M(−1) and a low detection limit (LOD) of 70 pM. Finally, the Y-MOF@S2@IL-6 hybrid platform allows the detection of oseltamivir with high sensitivity (K(sv) value is as high as 5.6 × 10(5) M(−1) and LOD is 54 nM), due to the fact that oseltamivir can disconnect the loop stem structure constructed by S2, leading to a strong quenching effect towards Y-MOF@S2@IL-6. The nature of the interactions between oseltamivir and Y-MOF has been elucidated using density functional theory calculations while the sensing mechanism for the dual detection of IL-6 and oseltamivir has been deciphered based on luminescence lifetime tests and confocal laser scanning microscopy.
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spelling pubmed-102078942023-05-25 A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs Wang, Xinrui Clavier, Gilles Zhang, Yan Batra, Kamal Xiao, Nanan Maurin, Guillaume Ding, Bin Tissot, Antoine Serre, Christian Chem Sci Chemistry COVID-19 has afflicted people's lives worldwide. Interleukin-6 (IL-6) is an important COVID-19 biomarker in human body fluids that can be used as a reference to monitor COVID-19 in real-time and therefore to reduce the risk of virus transmission. On the other hand, oseltamivir is a potential COVID-19 curing drug, but its overuse easily leads to hazardous side effects, calling for its real time monitoring in body fluids. For these purposes, a new yttrium metal–organic framework (Y-MOF) has been synthesized, in which the 5-(4-(imidazole-1-yl)phenyl)isophthalic linker contains a large aromatic backbone capable of strongly interacting with DNA sequences through π–π stacking interactions, which makes it appealing to build a unique sensor based on DNA functionalized MOFs. The MOF/DNA sequence hybrid luminescent sensing platform presents excellent optical properties associated with a high Förster resonance energy transfer (FRET) efficiency. Furthermore, to construct a dual emission sensing platform, a 5′-carboxylfluorescein (FAM) labeled DNA sequence (S2) with a stem-loop structure that can specifically interact with IL-6 has been associated with the Y-MOF. The resulting Y-MOF@S2 exhibits an efficient ratiometric detection of IL-6 in human body fluids with an extremely high K(sv) value 4.3 × 10(8) M(−1) and a low detection limit (LOD) of 70 pM. Finally, the Y-MOF@S2@IL-6 hybrid platform allows the detection of oseltamivir with high sensitivity (K(sv) value is as high as 5.6 × 10(5) M(−1) and LOD is 54 nM), due to the fact that oseltamivir can disconnect the loop stem structure constructed by S2, leading to a strong quenching effect towards Y-MOF@S2@IL-6. The nature of the interactions between oseltamivir and Y-MOF has been elucidated using density functional theory calculations while the sensing mechanism for the dual detection of IL-6 and oseltamivir has been deciphered based on luminescence lifetime tests and confocal laser scanning microscopy. The Royal Society of Chemistry 2023-04-19 /pmc/articles/PMC10207894/ /pubmed/37234896 http://dx.doi.org/10.1039/d3sc00106g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wang, Xinrui
Clavier, Gilles
Zhang, Yan
Batra, Kamal
Xiao, Nanan
Maurin, Guillaume
Ding, Bin
Tissot, Antoine
Serre, Christian
A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title_full A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title_fullStr A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title_full_unstemmed A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title_short A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs
title_sort mof/dna luminescent sensing platform for detection of potential covid-19 biomarkers and drugs
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10207894/
https://www.ncbi.nlm.nih.gov/pubmed/37234896
http://dx.doi.org/10.1039/d3sc00106g
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