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Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities

Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-...

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Autores principales: WANG, GANG, QIAN, MINGPING, JIAN, WEI, CHU, JUHANG, HUANG, YIXIANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tech Science Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208013/
https://www.ncbi.nlm.nih.gov/pubmed/37303741
http://dx.doi.org/10.32604/or.2022.028168
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author WANG, GANG
QIAN, MINGPING
JIAN, WEI
CHU, JUHANG
HUANG, YIXIANG
author_facet WANG, GANG
QIAN, MINGPING
JIAN, WEI
CHU, JUHANG
HUANG, YIXIANG
author_sort WANG, GANG
collection PubMed
description Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction, has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069 was highly expressed in breast cancer tissues and cells. The expression of has_circ_0000069 was associated with the five-year overall survival of patients. After silencing has_circ_0000069 in breast cancer cells, its expression reduced, and the ability of cell proliferation, migration, and invasion decreased. MiR-432 was verified as a targeting miRNA of has_circ_0000069. Has_circ_0000069 expression increased in breast cancer and was negatively related to patient’s prognosis. Has_circ_0000069 may facilitate breast cancer tumor progression by sponging miR-432. These findings revealed that has_circ_0000069 may be a biomarker for predicting prognosis and a therapeutic target for treating patients with breast cancer.
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spelling pubmed-102080132023-06-10 Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities WANG, GANG QIAN, MINGPING JIAN, WEI CHU, JUHANG HUANG, YIXIANG Oncol Res Article Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction, has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069 was highly expressed in breast cancer tissues and cells. The expression of has_circ_0000069 was associated with the five-year overall survival of patients. After silencing has_circ_0000069 in breast cancer cells, its expression reduced, and the ability of cell proliferation, migration, and invasion decreased. MiR-432 was verified as a targeting miRNA of has_circ_0000069. Has_circ_0000069 expression increased in breast cancer and was negatively related to patient’s prognosis. Has_circ_0000069 may facilitate breast cancer tumor progression by sponging miR-432. These findings revealed that has_circ_0000069 may be a biomarker for predicting prognosis and a therapeutic target for treating patients with breast cancer. Tech Science Press 2023-03-01 /pmc/articles/PMC10208013/ /pubmed/37303741 http://dx.doi.org/10.32604/or.2022.028168 Text en © 2023 Wang et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
WANG, GANG
QIAN, MINGPING
JIAN, WEI
CHU, JUHANG
HUANG, YIXIANG
Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title_full Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title_fullStr Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title_full_unstemmed Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title_short Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
title_sort has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208013/
https://www.ncbi.nlm.nih.gov/pubmed/37303741
http://dx.doi.org/10.32604/or.2022.028168
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