Cargando…

Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer

OBJECTIVES: The mitochondrial ribosomal protein L14 (MRPL14) is encoded by a nuclear gene and participates in mitochondrial protein translation. In this study, we aimed to investigate the role of MRPL14 in thyroid cancer. METHODS: We investigated the association between MRPL14 expression and clinico...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hae Jong, Nguyen, Quoc Khanh, Jung, Seung-Nam, Lim, Mi Ae, Oh, Chan, Piao, Yudan, Jin, YanLi, Kim, Ju-Hui, Kim, Young Il, Kang, Yea Eun, Chang, Jae Won, Won, Ho-Ryun, Koo, Bon Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Otorhinolaryngology-Head and Neck Surgery 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208851/
https://www.ncbi.nlm.nih.gov/pubmed/36822197
http://dx.doi.org/10.21053/ceo.2022.01760
_version_ 1785046759379042304
author Kim, Hae Jong
Nguyen, Quoc Khanh
Jung, Seung-Nam
Lim, Mi Ae
Oh, Chan
Piao, Yudan
Jin, YanLi
Kim, Ju-Hui
Kim, Young Il
Kang, Yea Eun
Chang, Jae Won
Won, Ho-Ryun
Koo, Bon Seok
author_facet Kim, Hae Jong
Nguyen, Quoc Khanh
Jung, Seung-Nam
Lim, Mi Ae
Oh, Chan
Piao, Yudan
Jin, YanLi
Kim, Ju-Hui
Kim, Young Il
Kang, Yea Eun
Chang, Jae Won
Won, Ho-Ryun
Koo, Bon Seok
author_sort Kim, Hae Jong
collection PubMed
description OBJECTIVES: The mitochondrial ribosomal protein L14 (MRPL14) is encoded by a nuclear gene and participates in mitochondrial protein translation. In this study, we aimed to investigate the role of MRPL14 in thyroid cancer. METHODS: We investigated the association between MRPL14 expression and clinicopathological features using The Cancer Genome Atlas (TCGA) and Chungnam National University Hospital (CNUH) databases. Functional studies of MRPL14, including proliferation, migration, invasion, mitochondrial oxidative phosphorylation and reactive oxygen species (ROS) production, were performed in papillary thyroid cancer (PTC) cell lines (B-CPAP and KTC-1). RESULTS: Based on the TCGA dataset, PTC tissues lost mitochondrial integrity and showed dysregulated expression of overall mitoribosomal proteins (MRPs) compared with normal thyroid tissues. Of 78 MRPs, MRPL14 was highly expressed in thyroid cancer tissues. MRPL14 overexpression was significantly associated with advanced tumor stage, extrathyroidal extension, and lymph node metastasis. MRPL14 increased cell proliferation of thyroid cancer and promoted cell migration via epithelial-mesenchymal transition-related proteins. Moreover, MRPL14 knockdown reduced the expression of oxidative phosphorylation complex IV (MTCO1) and increased the accumulation of ROS. Cotreatment with a ROS scavenger restored cell proliferation and migration, which had been reduced by MRPL14 knockdown, implying that ROS functions as a key regulator of the oncogenic effects of MRPL14 in thyroid cancer cells. CONCLUSION: Our findings indicate that MRPL14 may promote cell growth, migration, and invasion by modulating ROS in thyroid cancer cells.
format Online
Article
Text
id pubmed-10208851
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Korean Society of Otorhinolaryngology-Head and Neck Surgery
record_format MEDLINE/PubMed
spelling pubmed-102088512023-05-26 Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer Kim, Hae Jong Nguyen, Quoc Khanh Jung, Seung-Nam Lim, Mi Ae Oh, Chan Piao, Yudan Jin, YanLi Kim, Ju-Hui Kim, Young Il Kang, Yea Eun Chang, Jae Won Won, Ho-Ryun Koo, Bon Seok Clin Exp Otorhinolaryngol Original Article OBJECTIVES: The mitochondrial ribosomal protein L14 (MRPL14) is encoded by a nuclear gene and participates in mitochondrial protein translation. In this study, we aimed to investigate the role of MRPL14 in thyroid cancer. METHODS: We investigated the association between MRPL14 expression and clinicopathological features using The Cancer Genome Atlas (TCGA) and Chungnam National University Hospital (CNUH) databases. Functional studies of MRPL14, including proliferation, migration, invasion, mitochondrial oxidative phosphorylation and reactive oxygen species (ROS) production, were performed in papillary thyroid cancer (PTC) cell lines (B-CPAP and KTC-1). RESULTS: Based on the TCGA dataset, PTC tissues lost mitochondrial integrity and showed dysregulated expression of overall mitoribosomal proteins (MRPs) compared with normal thyroid tissues. Of 78 MRPs, MRPL14 was highly expressed in thyroid cancer tissues. MRPL14 overexpression was significantly associated with advanced tumor stage, extrathyroidal extension, and lymph node metastasis. MRPL14 increased cell proliferation of thyroid cancer and promoted cell migration via epithelial-mesenchymal transition-related proteins. Moreover, MRPL14 knockdown reduced the expression of oxidative phosphorylation complex IV (MTCO1) and increased the accumulation of ROS. Cotreatment with a ROS scavenger restored cell proliferation and migration, which had been reduced by MRPL14 knockdown, implying that ROS functions as a key regulator of the oncogenic effects of MRPL14 in thyroid cancer cells. CONCLUSION: Our findings indicate that MRPL14 may promote cell growth, migration, and invasion by modulating ROS in thyroid cancer cells. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2023-05 2023-02-23 /pmc/articles/PMC10208851/ /pubmed/36822197 http://dx.doi.org/10.21053/ceo.2022.01760 Text en Copyright © 2023 by Korean Society of Otorhinolaryngology-Head and Neck Surgery https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Hae Jong
Nguyen, Quoc Khanh
Jung, Seung-Nam
Lim, Mi Ae
Oh, Chan
Piao, Yudan
Jin, YanLi
Kim, Ju-Hui
Kim, Young Il
Kang, Yea Eun
Chang, Jae Won
Won, Ho-Ryun
Koo, Bon Seok
Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title_full Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title_fullStr Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title_full_unstemmed Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title_short Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer
title_sort mitochondrial ribosomal protein l14 promotes cell growth and invasion by modulating reactive oxygen species in thyroid cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208851/
https://www.ncbi.nlm.nih.gov/pubmed/36822197
http://dx.doi.org/10.21053/ceo.2022.01760
work_keys_str_mv AT kimhaejong mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT nguyenquockhanh mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT jungseungnam mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT limmiae mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT ohchan mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT piaoyudan mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT jinyanli mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT kimjuhui mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT kimyoungil mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT kangyeaeun mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT changjaewon mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT wonhoryun mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer
AT koobonseok mitochondrialribosomalproteinl14promotescellgrowthandinvasionbymodulatingreactiveoxygenspeciesinthyroidcancer