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Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss
OBJECTIVES: Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant fun...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208853/ https://www.ncbi.nlm.nih.gov/pubmed/36634670 http://dx.doi.org/10.21053/ceo.2022.01235 |
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author | Kim, Janghyun Lee, Bora Lee, Sungsu Kim, Joon-Tae Kim, Byeong C. Cho, Hyong-Ho |
author_facet | Kim, Janghyun Lee, Bora Lee, Sungsu Kim, Joon-Tae Kim, Byeong C. Cho, Hyong-Ho |
author_sort | Kim, Janghyun |
collection | PubMed |
description | OBJECTIVES: Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant function are associated with subsequent cochlear senescence in response to aging stress. Long non-coding RNA (lncRNA) has been reported to play important roles in various diseases. However, the function of lncRNA in ARHL remains unclear. In this study, we analyzed the common expression profiles of messenger RNA (mRNA) and lncRNA through ARHL-related RNA-sequencing datasets. METHODS: We selected and downloaded three different sets of RNA-sequencing data for ARHL. We performed differential expression analysis to find common mRNA and lncRNA profiles in the cochleae of aged mice compared to young mice. Gene Ontology (GO) analysis was used for functional exploration. Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate mRNAs and lncRNAs. In addition, we performed trans target prediction analysis with differentially expressed mRNAs and lncRNAs to understand the function of these mRNAs and lncRNAs in ARHL. RESULTS: We identified 112 common mRNAs and 10 common lncRNAs in the cochleae of aged mice compared to young mice. GO analysis showed that the 112 upregulated mRNAs were enriched in the defense response pathway. When we performed qRT-PCR with 1 mM H(2)O(2)-treated House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, the qRT-PCR results were consistent with the RNA-sequencing analysis data. lncRNA-mRNA networks were constructed using the 10 common lncRNAs and 112 common mRNAs in ARHL. CONCLUSION: Our study provides a comprehensive understanding of the common mRNA and lncRNA expression profiles in ARHL. Knowledge of ARHL-associated mRNAs and lncRNAs could be useful for better understanding ARHL and these mRNAs and lncRNAs might be a potential therapeutic target for preventing ARHL. |
format | Online Article Text |
id | pubmed-10208853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-102088532023-05-26 Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss Kim, Janghyun Lee, Bora Lee, Sungsu Kim, Joon-Tae Kim, Byeong C. Cho, Hyong-Ho Clin Exp Otorhinolaryngol Original Article OBJECTIVES: Age-related hearing loss (ARHL), or presbycusis, is caused by disorders of sensory hair cells and auditory neurons. Many studies have suggested that the accumulation of mitochondrial DNA damage, the production of reactive oxygen species, noise, inflammation, and decreased antioxidant function are associated with subsequent cochlear senescence in response to aging stress. Long non-coding RNA (lncRNA) has been reported to play important roles in various diseases. However, the function of lncRNA in ARHL remains unclear. In this study, we analyzed the common expression profiles of messenger RNA (mRNA) and lncRNA through ARHL-related RNA-sequencing datasets. METHODS: We selected and downloaded three different sets of RNA-sequencing data for ARHL. We performed differential expression analysis to find common mRNA and lncRNA profiles in the cochleae of aged mice compared to young mice. Gene Ontology (GO) analysis was used for functional exploration. Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate mRNAs and lncRNAs. In addition, we performed trans target prediction analysis with differentially expressed mRNAs and lncRNAs to understand the function of these mRNAs and lncRNAs in ARHL. RESULTS: We identified 112 common mRNAs and 10 common lncRNAs in the cochleae of aged mice compared to young mice. GO analysis showed that the 112 upregulated mRNAs were enriched in the defense response pathway. When we performed qRT-PCR with 1 mM H(2)O(2)-treated House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, the qRT-PCR results were consistent with the RNA-sequencing analysis data. lncRNA-mRNA networks were constructed using the 10 common lncRNAs and 112 common mRNAs in ARHL. CONCLUSION: Our study provides a comprehensive understanding of the common mRNA and lncRNA expression profiles in ARHL. Knowledge of ARHL-associated mRNAs and lncRNAs could be useful for better understanding ARHL and these mRNAs and lncRNAs might be a potential therapeutic target for preventing ARHL. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2023-05 2023-01-10 /pmc/articles/PMC10208853/ /pubmed/36634670 http://dx.doi.org/10.21053/ceo.2022.01235 Text en Copyright © 2023 by Korean Society of Otorhinolaryngology-Head and Neck Surgery https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Janghyun Lee, Bora Lee, Sungsu Kim, Joon-Tae Kim, Byeong C. Cho, Hyong-Ho Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title_full | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title_fullStr | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title_full_unstemmed | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title_short | Identification and Characterization of mRNA and lncRNA Expression Profiles in Age-Related Hearing Loss |
title_sort | identification and characterization of mrna and lncrna expression profiles in age-related hearing loss |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208853/ https://www.ncbi.nlm.nih.gov/pubmed/36634670 http://dx.doi.org/10.21053/ceo.2022.01235 |
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