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Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana
Human papillomavirus (HPV) E6 and E7 oncogene expression is essential for cervical carcinogenesis. Evidence exists that E6/E7 variants may have different transforming activities while the risk of HPV-16 variants (A/D) differs by race/ethnicity. We determined the type-specific diversity of HPV infect...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209332/ https://www.ncbi.nlm.nih.gov/pubmed/37179021 http://dx.doi.org/10.1016/j.tvr.2023.200261 |
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author | Kaba, Gladys Stevenson, Andrew Sakyi, Samuel Asamoah Konney, Thomas Okpoti Bhatia, Ramya Titiloye, Nicholas A. Oppong, Samuel A. Agyemang-Yeboah, Francis Cuschieri, Kate Graham, Sheila V. |
author_facet | Kaba, Gladys Stevenson, Andrew Sakyi, Samuel Asamoah Konney, Thomas Okpoti Bhatia, Ramya Titiloye, Nicholas A. Oppong, Samuel A. Agyemang-Yeboah, Francis Cuschieri, Kate Graham, Sheila V. |
author_sort | Kaba, Gladys |
collection | PubMed |
description | Human papillomavirus (HPV) E6 and E7 oncogene expression is essential for cervical carcinogenesis. Evidence exists that E6/E7 variants may have different transforming activities while the risk of HPV-16 variants (A/D) differs by race/ethnicity. We determined the type-specific diversity of HPV infection in women with high grade cervical disease or cervical cancer in Ghana and investigated naturally occurring E6/E7 DNA variants in this population. HPV genotyping was carried out on 207 cervical swab samples collected from women referred to a gynaecology clinic at two teaching hospitals in Ghana. HPV-16, HPV-18 and HPV-45 were detected in 41.9%, 23.3% and 16.3% of cases respectively. HPV-16 E6/E7 DNA sequencing was performed in 36 samples. Thirty samples contained E6/E7 variants of the HPV-16-B/C lineage. 21/36 samples were of the HPV-16C1 sublineage variant and all contained the E7 A647G(N29S) single nucleotide polymorphism (SNP). This study reveals the diversity of E6/E7 DNA and the dominance of HPV16 B/C variants in cervicovaginal HPV infection in Ghana. Type-specific HPV diversity analysis indicates that most Ghanaian cervical disease cases are vaccine preventable. The study provides an important baseline from which for the impact of vaccine and antivirals on clinically relevant HPV infection and associated disease can be measured. |
format | Online Article Text |
id | pubmed-10209332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-102093322023-05-26 Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana Kaba, Gladys Stevenson, Andrew Sakyi, Samuel Asamoah Konney, Thomas Okpoti Bhatia, Ramya Titiloye, Nicholas A. Oppong, Samuel A. Agyemang-Yeboah, Francis Cuschieri, Kate Graham, Sheila V. Tumour Virus Res Full Length Article Human papillomavirus (HPV) E6 and E7 oncogene expression is essential for cervical carcinogenesis. Evidence exists that E6/E7 variants may have different transforming activities while the risk of HPV-16 variants (A/D) differs by race/ethnicity. We determined the type-specific diversity of HPV infection in women with high grade cervical disease or cervical cancer in Ghana and investigated naturally occurring E6/E7 DNA variants in this population. HPV genotyping was carried out on 207 cervical swab samples collected from women referred to a gynaecology clinic at two teaching hospitals in Ghana. HPV-16, HPV-18 and HPV-45 were detected in 41.9%, 23.3% and 16.3% of cases respectively. HPV-16 E6/E7 DNA sequencing was performed in 36 samples. Thirty samples contained E6/E7 variants of the HPV-16-B/C lineage. 21/36 samples were of the HPV-16C1 sublineage variant and all contained the E7 A647G(N29S) single nucleotide polymorphism (SNP). This study reveals the diversity of E6/E7 DNA and the dominance of HPV16 B/C variants in cervicovaginal HPV infection in Ghana. Type-specific HPV diversity analysis indicates that most Ghanaian cervical disease cases are vaccine preventable. The study provides an important baseline from which for the impact of vaccine and antivirals on clinically relevant HPV infection and associated disease can be measured. Elsevier 2023-05-11 /pmc/articles/PMC10209332/ /pubmed/37179021 http://dx.doi.org/10.1016/j.tvr.2023.200261 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Full Length Article Kaba, Gladys Stevenson, Andrew Sakyi, Samuel Asamoah Konney, Thomas Okpoti Bhatia, Ramya Titiloye, Nicholas A. Oppong, Samuel A. Agyemang-Yeboah, Francis Cuschieri, Kate Graham, Sheila V. Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title | Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title_full | Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title_fullStr | Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title_full_unstemmed | Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title_short | Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana |
title_sort | diversity of cervicovaginal human papillomavirus (hpv) genotypes and naturally occurring e6/e7 dna polymorphisms of hpv-16 in ghana |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209332/ https://www.ncbi.nlm.nih.gov/pubmed/37179021 http://dx.doi.org/10.1016/j.tvr.2023.200261 |
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