Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization

The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene express...

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Detalles Bibliográficos
Autores principales: SEO, Hikari, MURATA, Shiro, ICHII, Osamu, NAMBA, Takashi, WIN, Shwe Yee, SATO, Takumi, OISHI, Eiji, TANENO, Akira, MAEKAWA, Naoya, OKAGAWA, Tomohiro, KONNAI, Satoru, OHASHI, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2023
Materias:
Avian Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209471/
https://www.ncbi.nlm.nih.gov/pubmed/36927857
http://dx.doi.org/10.1292/jvms.22-0544
Descripción
Sumario:The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene expression in PRM. We analyzed the mRNA expression of Dermanyssus gallinae cathepsin D-1 (Dg-CatD-1) and Dermanyssus gallinae cystatin (Dg-Cys). RNAscope ISH analysis revealed that mRNA expression of Dg-CatD-1 was observed in the digestive tract, and Dg-Cystatin mRNA was expressed in the ovaries in addition to the digestive tract. RNAscope ISH could be applicable for the analysis of gene expression in each tissue of PRM and is an effective method to investigate the characteristics of target genes.

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