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Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity

Copper oxide nanoparticles (CuO‐NPs) were functionalized with specific antibodies to target their antibacterial activity against Gram‐positive or Gram‐negative bacteria. The CuO‐NPs were covalently functionalized to cover their surface with specific antibodies. The differently prepared CuO‐NPs were...

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Autores principales: Ontiveros‐Robles, Jorge A., Villanueva‐Flores, Francisca, Juarez‐Moreno, Karla, Simakov, Andrey, Vazquez‐Duhalt, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209517/
https://www.ncbi.nlm.nih.gov/pubmed/37226371
http://dx.doi.org/10.1002/open.202200241
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author Ontiveros‐Robles, Jorge A.
Villanueva‐Flores, Francisca
Juarez‐Moreno, Karla
Simakov, Andrey
Vazquez‐Duhalt, Rafael
author_facet Ontiveros‐Robles, Jorge A.
Villanueva‐Flores, Francisca
Juarez‐Moreno, Karla
Simakov, Andrey
Vazquez‐Duhalt, Rafael
author_sort Ontiveros‐Robles, Jorge A.
collection PubMed
description Copper oxide nanoparticles (CuO‐NPs) were functionalized with specific antibodies to target their antibacterial activity against Gram‐positive or Gram‐negative bacteria. The CuO‐NPs were covalently functionalized to cover their surface with specific antibodies. The differently prepared CuO‐NPs were characterized by X‐ray diffraction, transmission electron microscopy and dynamic light scattering. The antibacterial activities of the unmodified CuO‐NPs and the antibody‐functionalized nanoparticles (CuO‐NP‐AbGram(−) and CuO‐NP‐AbGram(+)) were determined for both Gram‐negative Escherichia coli and Gram‐positive Bacillus subtilis bacteria. The antibody‐functionalized NPs showed a differential increase of their antibacterial activity according to the specific antibody. The CuO‐NP‐AbGram(−) in E. coli showed reduced half maximal inhibitory concentration (IC(50)) and minimum inhibitory concentration (MIC) values when compared with unfunctionalized CuO‐NPs. On the other hand, the CuO‐NP‐AbGram(+) also showed reduced IC(50) and MIC values in B. subtilis, when compared with non‐functionalized CuO‐NPs. Thus, the functionalized CuO nanoparticles with specific antibodies showed enhanced specificity of their antibacterial activity. The advantages of “smart” antibiotic nanoparticles are discussed.
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spelling pubmed-102095172023-05-26 Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity Ontiveros‐Robles, Jorge A. Villanueva‐Flores, Francisca Juarez‐Moreno, Karla Simakov, Andrey Vazquez‐Duhalt, Rafael ChemistryOpen Research Articles Copper oxide nanoparticles (CuO‐NPs) were functionalized with specific antibodies to target their antibacterial activity against Gram‐positive or Gram‐negative bacteria. The CuO‐NPs were covalently functionalized to cover their surface with specific antibodies. The differently prepared CuO‐NPs were characterized by X‐ray diffraction, transmission electron microscopy and dynamic light scattering. The antibacterial activities of the unmodified CuO‐NPs and the antibody‐functionalized nanoparticles (CuO‐NP‐AbGram(−) and CuO‐NP‐AbGram(+)) were determined for both Gram‐negative Escherichia coli and Gram‐positive Bacillus subtilis bacteria. The antibody‐functionalized NPs showed a differential increase of their antibacterial activity according to the specific antibody. The CuO‐NP‐AbGram(−) in E. coli showed reduced half maximal inhibitory concentration (IC(50)) and minimum inhibitory concentration (MIC) values when compared with unfunctionalized CuO‐NPs. On the other hand, the CuO‐NP‐AbGram(+) also showed reduced IC(50) and MIC values in B. subtilis, when compared with non‐functionalized CuO‐NPs. Thus, the functionalized CuO nanoparticles with specific antibodies showed enhanced specificity of their antibacterial activity. The advantages of “smart” antibiotic nanoparticles are discussed. John Wiley and Sons Inc. 2023-05-24 /pmc/articles/PMC10209517/ /pubmed/37226371 http://dx.doi.org/10.1002/open.202200241 Text en © 2023 The Authors. Published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ontiveros‐Robles, Jorge A.
Villanueva‐Flores, Francisca
Juarez‐Moreno, Karla
Simakov, Andrey
Vazquez‐Duhalt, Rafael
Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title_full Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title_fullStr Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title_full_unstemmed Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title_short Antibody‐Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity
title_sort antibody‐functionalized copper oxide nanoparticles with targeted antibacterial activity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209517/
https://www.ncbi.nlm.nih.gov/pubmed/37226371
http://dx.doi.org/10.1002/open.202200241
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