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Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study
Reactive and highly reactive oxygen species (ROS and hROS) produced by white blood cells are essential for innate immunity; however, they may cause oxidative stress in the host. We developed systems for simultaneously monitoring ROS and hROS, i.e., superoxide radicals (O(2)(•−)) and hypochlorite ion...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Society for Free Radical Research Japan
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209599/ https://www.ncbi.nlm.nih.gov/pubmed/37251959 http://dx.doi.org/10.3164/jcbn.22-109 |
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author | Takeuchi, Kozo Kazumura, Kimiko Yoshida, Akihiro Furuta, Tappei Hayashi, Kazunori Nagai, Masashi Hatano, Yukiko Naito, Michitaka Matsushita, Etsushi |
author_facet | Takeuchi, Kozo Kazumura, Kimiko Yoshida, Akihiro Furuta, Tappei Hayashi, Kazunori Nagai, Masashi Hatano, Yukiko Naito, Michitaka Matsushita, Etsushi |
author_sort | Takeuchi, Kozo |
collection | PubMed |
description | Reactive and highly reactive oxygen species (ROS and hROS) produced by white blood cells are essential for innate immunity; however, they may cause oxidative stress in the host. We developed systems for simultaneously monitoring ROS and hROS, i.e., superoxide radicals (O(2)(•−)) and hypochlorite ions (OCl(−)) secreted from stimulated white blood cells in a few microliters of whole blood. We previously reported on the evaluation of healthy volunteers’ blood using the developed system; however, whether patients’ blood can be assessed remains unclear. Here, we report a pilot study of 30 cases (28 patients) with peripheral arterial disease, in whom we measured the ROS and hROS levels before and approximately one month after endovascular treatment (EVT) using the system (CFL-H2200) that we developed. At approximately the same time points, physiological indices of blood vessels, oxidative stress markers, and standard clinical parameters in the blood were also monitored. The ankle-brachial index, a diagnostic tool for peripheral arterial disease, was significantly improved after EVT (p<0.001). The ROS-hROS ratio, low-density lipoprotein cholesterol, and hematocrit levels were decreased after EVT (p<0.05), while triglyceride and lymphocyte levels were increased after EVT (p<0.05). The correlations between the study parameters were also analyzed. |
format | Online Article Text |
id | pubmed-10209599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-102095992023-05-26 Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study Takeuchi, Kozo Kazumura, Kimiko Yoshida, Akihiro Furuta, Tappei Hayashi, Kazunori Nagai, Masashi Hatano, Yukiko Naito, Michitaka Matsushita, Etsushi J Clin Biochem Nutr Original Article Reactive and highly reactive oxygen species (ROS and hROS) produced by white blood cells are essential for innate immunity; however, they may cause oxidative stress in the host. We developed systems for simultaneously monitoring ROS and hROS, i.e., superoxide radicals (O(2)(•−)) and hypochlorite ions (OCl(−)) secreted from stimulated white blood cells in a few microliters of whole blood. We previously reported on the evaluation of healthy volunteers’ blood using the developed system; however, whether patients’ blood can be assessed remains unclear. Here, we report a pilot study of 30 cases (28 patients) with peripheral arterial disease, in whom we measured the ROS and hROS levels before and approximately one month after endovascular treatment (EVT) using the system (CFL-H2200) that we developed. At approximately the same time points, physiological indices of blood vessels, oxidative stress markers, and standard clinical parameters in the blood were also monitored. The ankle-brachial index, a diagnostic tool for peripheral arterial disease, was significantly improved after EVT (p<0.001). The ROS-hROS ratio, low-density lipoprotein cholesterol, and hematocrit levels were decreased after EVT (p<0.05), while triglyceride and lymphocyte levels were increased after EVT (p<0.05). The correlations between the study parameters were also analyzed. the Society for Free Radical Research Japan 2023-05 2023-03-04 /pmc/articles/PMC10209599/ /pubmed/37251959 http://dx.doi.org/10.3164/jcbn.22-109 Text en Copyright © 2023 JCBN https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Takeuchi, Kozo Kazumura, Kimiko Yoshida, Akihiro Furuta, Tappei Hayashi, Kazunori Nagai, Masashi Hatano, Yukiko Naito, Michitaka Matsushita, Etsushi Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title | Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title_full | Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title_fullStr | Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title_full_unstemmed | Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title_short | Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
title_sort | comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209599/ https://www.ncbi.nlm.nih.gov/pubmed/37251959 http://dx.doi.org/10.3164/jcbn.22-109 |
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