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Quantitative elemental imaging in eukaryotic algae
All organisms, fundamentally, are made from the same raw material, namely the elements of the periodic table. Biochemical diversity is achieved by how these elements are utilized, for what purpose, and in which physical location. Determining elemental distributions, especially those of trace element...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209819/ https://www.ncbi.nlm.nih.gov/pubmed/37186252 http://dx.doi.org/10.1093/mtomcs/mfad025 |
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author | Schmollinger, Stefan Chen, Si Merchant, Sabeeha S |
author_facet | Schmollinger, Stefan Chen, Si Merchant, Sabeeha S |
author_sort | Schmollinger, Stefan |
collection | PubMed |
description | All organisms, fundamentally, are made from the same raw material, namely the elements of the periodic table. Biochemical diversity is achieved by how these elements are utilized, for what purpose, and in which physical location. Determining elemental distributions, especially those of trace elements that facilitate metabolism as cofactors in the active centers of essential enzymes, can determine the state of metabolism, the nutritional status, or the developmental stage of an organism. Photosynthetic eukaryotes, especially algae, are excellent subjects for quantitative analysis of elemental distribution. These microbes utilize unique metabolic pathways that require various trace nutrients at their core to enable their operation. Photosynthetic microbes also have important environmental roles as primary producers in habitats with limited nutrient supplies or toxin contaminations. Accordingly, photosynthetic eukaryotes are of great interest for biotechnological exploitation, carbon sequestration, and bioremediation, with many of the applications involving various trace elements and consequently affecting their quota and intracellular distribution. A number of diverse applications were developed for elemental imaging, allowing subcellular resolution, with X-ray fluorescence microscopy (XFM, XRF) being at the forefront, enabling quantitative descriptions of intact cells in a non-destructive method. This Tutorial Review summarizes the workflow of a quantitative, single-cell elemental distribution analysis of a eukaryotic alga using XFM. |
format | Online Article Text |
id | pubmed-10209819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-102098192023-06-27 Quantitative elemental imaging in eukaryotic algae Schmollinger, Stefan Chen, Si Merchant, Sabeeha S Metallomics Tutorial Review All organisms, fundamentally, are made from the same raw material, namely the elements of the periodic table. Biochemical diversity is achieved by how these elements are utilized, for what purpose, and in which physical location. Determining elemental distributions, especially those of trace elements that facilitate metabolism as cofactors in the active centers of essential enzymes, can determine the state of metabolism, the nutritional status, or the developmental stage of an organism. Photosynthetic eukaryotes, especially algae, are excellent subjects for quantitative analysis of elemental distribution. These microbes utilize unique metabolic pathways that require various trace nutrients at their core to enable their operation. Photosynthetic microbes also have important environmental roles as primary producers in habitats with limited nutrient supplies or toxin contaminations. Accordingly, photosynthetic eukaryotes are of great interest for biotechnological exploitation, carbon sequestration, and bioremediation, with many of the applications involving various trace elements and consequently affecting their quota and intracellular distribution. A number of diverse applications were developed for elemental imaging, allowing subcellular resolution, with X-ray fluorescence microscopy (XFM, XRF) being at the forefront, enabling quantitative descriptions of intact cells in a non-destructive method. This Tutorial Review summarizes the workflow of a quantitative, single-cell elemental distribution analysis of a eukaryotic alga using XFM. Oxford University Press 2023-04-25 /pmc/articles/PMC10209819/ /pubmed/37186252 http://dx.doi.org/10.1093/mtomcs/mfad025 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Tutorial Review Schmollinger, Stefan Chen, Si Merchant, Sabeeha S Quantitative elemental imaging in eukaryotic algae |
title | Quantitative elemental imaging in eukaryotic algae |
title_full | Quantitative elemental imaging in eukaryotic algae |
title_fullStr | Quantitative elemental imaging in eukaryotic algae |
title_full_unstemmed | Quantitative elemental imaging in eukaryotic algae |
title_short | Quantitative elemental imaging in eukaryotic algae |
title_sort | quantitative elemental imaging in eukaryotic algae |
topic | Tutorial Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209819/ https://www.ncbi.nlm.nih.gov/pubmed/37186252 http://dx.doi.org/10.1093/mtomcs/mfad025 |
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