Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies

[Image: see text] This study reports an impact of structure (XRPD, FT-IR) and surface morphology (SEM-EDS) of imatinib-functionalized galactose hydrogels, loaded and unloaded with nHAp, on osteosarcoma cell (Saos-2 and U-2OS) viability, levels of free oxygen radicals, and nitric oxide, levels of BCL...

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Autores principales: Sobierajska, Paulina, Wiatrak, Benita, Jawien, Paulina, Janeczek, Maciej, Wiglusz, Katarzyna, Szeląg, Adam, Wiglusz, Rafal J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210190/
https://www.ncbi.nlm.nih.gov/pubmed/37251195
http://dx.doi.org/10.1021/acsomega.3c00986
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author Sobierajska, Paulina
Wiatrak, Benita
Jawien, Paulina
Janeczek, Maciej
Wiglusz, Katarzyna
Szeląg, Adam
Wiglusz, Rafal J.
author_facet Sobierajska, Paulina
Wiatrak, Benita
Jawien, Paulina
Janeczek, Maciej
Wiglusz, Katarzyna
Szeląg, Adam
Wiglusz, Rafal J.
author_sort Sobierajska, Paulina
collection PubMed
description [Image: see text] This study reports an impact of structure (XRPD, FT-IR) and surface morphology (SEM-EDS) of imatinib-functionalized galactose hydrogels, loaded and unloaded with nHAp, on osteosarcoma cell (Saos-2 and U-2OS) viability, levels of free oxygen radicals, and nitric oxide, levels of BCL-2, p53, and caspase 3 and 9, as well as glycoprotein-P activity. It was investigated how the rough surface of the crystalline hydroxyapatite-modified hydrogel affected amorphous imatinib (IM) release. The imatinib drug effect on cell cultures has been demonstrated in different forms of administration—directly to the culture or the hydrogels. Administration of IM and hydrogel composites could be expected to reduce the risk of multidrug resistance development by inhibiting P(gp).
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spelling pubmed-102101902023-05-26 Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies Sobierajska, Paulina Wiatrak, Benita Jawien, Paulina Janeczek, Maciej Wiglusz, Katarzyna Szeląg, Adam Wiglusz, Rafal J. ACS Omega [Image: see text] This study reports an impact of structure (XRPD, FT-IR) and surface morphology (SEM-EDS) of imatinib-functionalized galactose hydrogels, loaded and unloaded with nHAp, on osteosarcoma cell (Saos-2 and U-2OS) viability, levels of free oxygen radicals, and nitric oxide, levels of BCL-2, p53, and caspase 3 and 9, as well as glycoprotein-P activity. It was investigated how the rough surface of the crystalline hydroxyapatite-modified hydrogel affected amorphous imatinib (IM) release. The imatinib drug effect on cell cultures has been demonstrated in different forms of administration—directly to the culture or the hydrogels. Administration of IM and hydrogel composites could be expected to reduce the risk of multidrug resistance development by inhibiting P(gp). American Chemical Society 2023-05-09 /pmc/articles/PMC10210190/ /pubmed/37251195 http://dx.doi.org/10.1021/acsomega.3c00986 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Sobierajska, Paulina
Wiatrak, Benita
Jawien, Paulina
Janeczek, Maciej
Wiglusz, Katarzyna
Szeląg, Adam
Wiglusz, Rafal J.
Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title_full Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title_fullStr Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title_full_unstemmed Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title_short Imatinib-Functionalized Galactose Hydrogels Loaded with Nanohydroxyapatite as a Drug Delivery System for Osteosarcoma: In Vitro Studies
title_sort imatinib-functionalized galactose hydrogels loaded with nanohydroxyapatite as a drug delivery system for osteosarcoma: in vitro studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210190/
https://www.ncbi.nlm.nih.gov/pubmed/37251195
http://dx.doi.org/10.1021/acsomega.3c00986
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