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Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model

[Image: see text] Due to the presence of the steroidal glycoalkaloid solanine, the potato was chosen as Germany’s poisonous plant of the year 2022. Steroidal glycoalkaloids are secondary plant metabolites which have been reported to induce toxic as well as beneficial health effects. Nevertheless, da...

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Autores principales: Kasimir, Matthias, Wolbeck, Alessa, Behrens, Matthias, Humpf, Hans-Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210216/
https://www.ncbi.nlm.nih.gov/pubmed/37251124
http://dx.doi.org/10.1021/acsomega.3c01990
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author Kasimir, Matthias
Wolbeck, Alessa
Behrens, Matthias
Humpf, Hans-Ulrich
author_facet Kasimir, Matthias
Wolbeck, Alessa
Behrens, Matthias
Humpf, Hans-Ulrich
author_sort Kasimir, Matthias
collection PubMed
description [Image: see text] Due to the presence of the steroidal glycoalkaloid solanine, the potato was chosen as Germany’s poisonous plant of the year 2022. Steroidal glycoalkaloids are secondary plant metabolites which have been reported to induce toxic as well as beneficial health effects. Nevertheless, data regarding occurrence, toxicokinetics, and metabolism of steroidal glycoalkaloids is scarce, and substantially more research is required for a proper risk assessment. Therefore, the intestinal metabolism of solanine, chaconine, solasonine, solamargine, and tomatine was investigated using the ex vivo pig cecum model. All steroidal glycoalkaloids were degraded by the porcine intestinal microbiota, releasing the respective aglycon. Furthermore, the hydrolysis rate was strongly dependent on the linked carbohydrate side chain. Solanine and solasonine, which are linked to a solatriose, were metabolized significantly faster than the chaconine and solamargin, which are linked to a chacotriose. In addition, stepwise cleavage of the carbohydrate side chain and the formation of β- and γ-intermediates were detected by HPLC–HRMS. The results provide valuable insights into the intestinal metabolism of selected steroidal glycoalkaloids and help to reduce uncertainties and improve risk assessment.
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spelling pubmed-102102162023-05-26 Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model Kasimir, Matthias Wolbeck, Alessa Behrens, Matthias Humpf, Hans-Ulrich ACS Omega [Image: see text] Due to the presence of the steroidal glycoalkaloid solanine, the potato was chosen as Germany’s poisonous plant of the year 2022. Steroidal glycoalkaloids are secondary plant metabolites which have been reported to induce toxic as well as beneficial health effects. Nevertheless, data regarding occurrence, toxicokinetics, and metabolism of steroidal glycoalkaloids is scarce, and substantially more research is required for a proper risk assessment. Therefore, the intestinal metabolism of solanine, chaconine, solasonine, solamargine, and tomatine was investigated using the ex vivo pig cecum model. All steroidal glycoalkaloids were degraded by the porcine intestinal microbiota, releasing the respective aglycon. Furthermore, the hydrolysis rate was strongly dependent on the linked carbohydrate side chain. Solanine and solasonine, which are linked to a solatriose, were metabolized significantly faster than the chaconine and solamargin, which are linked to a chacotriose. In addition, stepwise cleavage of the carbohydrate side chain and the formation of β- and γ-intermediates were detected by HPLC–HRMS. The results provide valuable insights into the intestinal metabolism of selected steroidal glycoalkaloids and help to reduce uncertainties and improve risk assessment. American Chemical Society 2023-05-12 /pmc/articles/PMC10210216/ /pubmed/37251124 http://dx.doi.org/10.1021/acsomega.3c01990 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kasimir, Matthias
Wolbeck, Alessa
Behrens, Matthias
Humpf, Hans-Ulrich
Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title_full Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title_fullStr Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title_full_unstemmed Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title_short Intestinal Metabolism of Selected Steroidal Glycoalkaloids in the Pig Cecum Model
title_sort intestinal metabolism of selected steroidal glycoalkaloids in the pig cecum model
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210216/
https://www.ncbi.nlm.nih.gov/pubmed/37251124
http://dx.doi.org/10.1021/acsomega.3c01990
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