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Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate

[Image: see text] There is a growing concern that the increasing concentration of CO(2) in the atmosphere contributes to a potential negative impact on global climate change. To deal with this problem, developing a set of innovative, practical technologies is essential. In the present study, maximiz...

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Autores principales: Asadi, Vahideh, Marandi, Afsaneh, Kardanpour, Reihaneh, Tangestaninejad, Shahram, Moghadam, Majid, Mirkhani, Valiollah, Mohammadpoor-Baltork, Iraj, Mirzaei, Razieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210226/
https://www.ncbi.nlm.nih.gov/pubmed/37251154
http://dx.doi.org/10.1021/acsomega.3c00691
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author Asadi, Vahideh
Marandi, Afsaneh
Kardanpour, Reihaneh
Tangestaninejad, Shahram
Moghadam, Majid
Mirkhani, Valiollah
Mohammadpoor-Baltork, Iraj
Mirzaei, Razieh
author_facet Asadi, Vahideh
Marandi, Afsaneh
Kardanpour, Reihaneh
Tangestaninejad, Shahram
Moghadam, Majid
Mirkhani, Valiollah
Mohammadpoor-Baltork, Iraj
Mirzaei, Razieh
author_sort Asadi, Vahideh
collection PubMed
description [Image: see text] There is a growing concern that the increasing concentration of CO(2) in the atmosphere contributes to a potential negative impact on global climate change. To deal with this problem, developing a set of innovative, practical technologies is essential. In the present study, maximizing the CO(2) utilization and precipitation as CaCO(3) was evaluated. In this manner, bovine carbonic anhydrase (BCA) was embedded into the microporous zeolite imidazolate framework, ZIF-8, via physical absorption and encapsulation. Running as crystal seeds, these nanocomposites (enzyme-embedded MOFs) were in situ grown on the cross-linked electrospun polyvinyl alcohol (CPVA). The prepared composites displayed much higher stability against denaturants, high temperatures, and acidic media than free BCA, and BCA immobilized into or on ZIF-8. During 37 days of storage period study, BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA maintained more than 99 and 75% of their initial activity, respectively. The composition of BCA@ZIF-8 and BCA/ZIF-8 with CPVA improved stability for consecutive usage in recovery reactions, recycling easiness, and greater control over the catalytic process. The amounts of calcium carbonate obtained by one mg each of fresh BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA were 55.45 and 49.15 mg, respectively. The precipitated calcium carbonate by BCA@ZIF-8/CPVA reached 64.8% of the initial run, while this amount was 43.6% for BCA/ZIF-8/CPVA after eight cycles. These results indicated that the BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA fibers could be efficiently applied to CO(2) sequestration.
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spelling pubmed-102102262023-05-26 Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate Asadi, Vahideh Marandi, Afsaneh Kardanpour, Reihaneh Tangestaninejad, Shahram Moghadam, Majid Mirkhani, Valiollah Mohammadpoor-Baltork, Iraj Mirzaei, Razieh ACS Omega [Image: see text] There is a growing concern that the increasing concentration of CO(2) in the atmosphere contributes to a potential negative impact on global climate change. To deal with this problem, developing a set of innovative, practical technologies is essential. In the present study, maximizing the CO(2) utilization and precipitation as CaCO(3) was evaluated. In this manner, bovine carbonic anhydrase (BCA) was embedded into the microporous zeolite imidazolate framework, ZIF-8, via physical absorption and encapsulation. Running as crystal seeds, these nanocomposites (enzyme-embedded MOFs) were in situ grown on the cross-linked electrospun polyvinyl alcohol (CPVA). The prepared composites displayed much higher stability against denaturants, high temperatures, and acidic media than free BCA, and BCA immobilized into or on ZIF-8. During 37 days of storage period study, BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA maintained more than 99 and 75% of their initial activity, respectively. The composition of BCA@ZIF-8 and BCA/ZIF-8 with CPVA improved stability for consecutive usage in recovery reactions, recycling easiness, and greater control over the catalytic process. The amounts of calcium carbonate obtained by one mg each of fresh BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA were 55.45 and 49.15 mg, respectively. The precipitated calcium carbonate by BCA@ZIF-8/CPVA reached 64.8% of the initial run, while this amount was 43.6% for BCA/ZIF-8/CPVA after eight cycles. These results indicated that the BCA@ZIF-8/CPVA and BCA/ZIF-8/CPVA fibers could be efficiently applied to CO(2) sequestration. American Chemical Society 2023-05-09 /pmc/articles/PMC10210226/ /pubmed/37251154 http://dx.doi.org/10.1021/acsomega.3c00691 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Asadi, Vahideh
Marandi, Afsaneh
Kardanpour, Reihaneh
Tangestaninejad, Shahram
Moghadam, Majid
Mirkhani, Valiollah
Mohammadpoor-Baltork, Iraj
Mirzaei, Razieh
Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title_full Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title_fullStr Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title_full_unstemmed Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title_short Carbonic Anhydrase-Embedded ZIF-8 Electrospun PVA Fibers as an Excellent Biocatalyst Candidate
title_sort carbonic anhydrase-embedded zif-8 electrospun pva fibers as an excellent biocatalyst candidate
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210226/
https://www.ncbi.nlm.nih.gov/pubmed/37251154
http://dx.doi.org/10.1021/acsomega.3c00691
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