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Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity

BACKGROUND: Macleaya cordata is a traditional medicinal herb, and it has high tolerance and accumulation ability to heavy metals, which make it a good candidate species for studying phytoremediation. The objectives of this study were to investigate response and tolerance of M. cordata to lead (Pb) t...

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Autores principales: Zhang, Hongxiao, Hu, Linfeng, Du, Xinlong, Sun, Xijing, Wang, Ting, Mu, Zhiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210377/
https://www.ncbi.nlm.nih.gov/pubmed/37226137
http://dx.doi.org/10.1186/s12864-023-09378-2
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author Zhang, Hongxiao
Hu, Linfeng
Du, Xinlong
Sun, Xijing
Wang, Ting
Mu, Zhiying
author_facet Zhang, Hongxiao
Hu, Linfeng
Du, Xinlong
Sun, Xijing
Wang, Ting
Mu, Zhiying
author_sort Zhang, Hongxiao
collection PubMed
description BACKGROUND: Macleaya cordata is a traditional medicinal herb, and it has high tolerance and accumulation ability to heavy metals, which make it a good candidate species for studying phytoremediation. The objectives of this study were to investigate response and tolerance of M. cordata to lead (Pb) toxicity based on comparative analysis of transcriptome and proteome. RESULTS: In this study, the seedlings of M. cordata cultured in Hoagland solution were treated with 100 µmol·L(− 1) Pb for 1 day (Pb 1d) or 7 days (Pb 7d), subsequently leaves of M. cordata were taken for the determination of Pb accumulation and hydrogen peroxide production (H(2)O(2)), meanwhile a total number of 223 significantly differentially expressed genes (DEGs) and 296 differentially expressed proteins (DEPs) were screened between control and Pb treatments. The results showed leaves of M. cordata had a special mechanism to maintain Pb at an appropriate level. Firstly, some DEGs were iron (Fe) deficiency-induced transporters, for example, genes of vacuolar iron transporter and three ABC transporter I family numbers were upregulated by Pb, which can maintain Fe homeostasis in cytoplasm or chloroplast. In addition, five genes of calcium (Ca(2+)) binding proteins were downregulated in Pb 1d, which may regulate cytoplasmic Ca(2+) concentration and H(2)O(2) signaling pathway. On the other hand, the cysteine synthase upregulated, glutathione S-transferase downregulated and glutathione reductase downregulated in Pb 7d can cause reduced glutathione accumulation and decrease Pb detoxification in leaves. Furthermore, DEPs of eight chlorophyll a/b binding proteins, five ATPases and eight ribosomal proteins can play a pivotal role on chloroplast turnover and ATP metabolism. CONCLUSIONS: Our results suggest that the proteins involved in Fe homeostasis and chloroplast turnover in mesophyll cells may play key roles in tolerance of M. cordata to Pb. This study offers some novel insights into Pb tolerance mechanism of plants, and the potential valuable for environmental remediation of this important medicinal plant. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09378-2.
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spelling pubmed-102103772023-05-26 Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity Zhang, Hongxiao Hu, Linfeng Du, Xinlong Sun, Xijing Wang, Ting Mu, Zhiying BMC Genomics Research BACKGROUND: Macleaya cordata is a traditional medicinal herb, and it has high tolerance and accumulation ability to heavy metals, which make it a good candidate species for studying phytoremediation. The objectives of this study were to investigate response and tolerance of M. cordata to lead (Pb) toxicity based on comparative analysis of transcriptome and proteome. RESULTS: In this study, the seedlings of M. cordata cultured in Hoagland solution were treated with 100 µmol·L(− 1) Pb for 1 day (Pb 1d) or 7 days (Pb 7d), subsequently leaves of M. cordata were taken for the determination of Pb accumulation and hydrogen peroxide production (H(2)O(2)), meanwhile a total number of 223 significantly differentially expressed genes (DEGs) and 296 differentially expressed proteins (DEPs) were screened between control and Pb treatments. The results showed leaves of M. cordata had a special mechanism to maintain Pb at an appropriate level. Firstly, some DEGs were iron (Fe) deficiency-induced transporters, for example, genes of vacuolar iron transporter and three ABC transporter I family numbers were upregulated by Pb, which can maintain Fe homeostasis in cytoplasm or chloroplast. In addition, five genes of calcium (Ca(2+)) binding proteins were downregulated in Pb 1d, which may regulate cytoplasmic Ca(2+) concentration and H(2)O(2) signaling pathway. On the other hand, the cysteine synthase upregulated, glutathione S-transferase downregulated and glutathione reductase downregulated in Pb 7d can cause reduced glutathione accumulation and decrease Pb detoxification in leaves. Furthermore, DEPs of eight chlorophyll a/b binding proteins, five ATPases and eight ribosomal proteins can play a pivotal role on chloroplast turnover and ATP metabolism. CONCLUSIONS: Our results suggest that the proteins involved in Fe homeostasis and chloroplast turnover in mesophyll cells may play key roles in tolerance of M. cordata to Pb. This study offers some novel insights into Pb tolerance mechanism of plants, and the potential valuable for environmental remediation of this important medicinal plant. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09378-2. BioMed Central 2023-05-24 /pmc/articles/PMC10210377/ /pubmed/37226137 http://dx.doi.org/10.1186/s12864-023-09378-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Hongxiao
Hu, Linfeng
Du, Xinlong
Sun, Xijing
Wang, Ting
Mu, Zhiying
Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title_full Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title_fullStr Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title_full_unstemmed Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title_short Physiological and molecular response and tolerance of Macleaya cordata to lead toxicity
title_sort physiological and molecular response and tolerance of macleaya cordata to lead toxicity
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210377/
https://www.ncbi.nlm.nih.gov/pubmed/37226137
http://dx.doi.org/10.1186/s12864-023-09378-2
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