Cargando…
A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. H...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210397/ https://www.ncbi.nlm.nih.gov/pubmed/37226181 http://dx.doi.org/10.1186/s13071-023-05787-0 |
_version_ | 1785047059058917376 |
---|---|
author | Zhang, Hongbo Zhao, Chunyan Zhang, Xichen Li, Jianhua Gong, Pengtao Wang, Xiaocen Li, Xin Wang, Xin Zhang, Xu Cheng, Shuqin Yue, Taotao Zhang, Nan |
author_facet | Zhang, Hongbo Zhao, Chunyan Zhang, Xichen Li, Jianhua Gong, Pengtao Wang, Xiaocen Li, Xin Wang, Xin Zhang, Xu Cheng, Shuqin Yue, Taotao Zhang, Nan |
author_sort | Zhang, Hongbo |
collection | PubMed |
description | BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated. METHODS: To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction and colocalization in Giardia trophozoites were examined by using Duolink proximal ligation assay (Duolink PLA). RESULTS: From Y2H screen, the Giardia chaperone protein, Giardia DnaJ (GdDnaJ), was identified as a new binding partner for GLV RdRp. The direct interaction between GdDnaJ and GLV RdRp was verified via GST pull-down, co-immunoprecipitation and BiFC. In addition, colocalization and in vivo interaction between GdDnaJ and RdRp in Giardia trophozoites were confirmed by Duolink PLA. Further analysis revealed that KNK437, the inhibitor of GdDnaJ, can significantly reduce the replication of GLVs and the proliferation of Giardia. CONCLUSION: Taken together, our results suggested a potential role of GdDnaJ in regulating Giardia proliferation and GLV replication through interaction with GLV RdRp. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05787-0. |
format | Online Article Text |
id | pubmed-10210397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102103972023-05-26 A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication Zhang, Hongbo Zhao, Chunyan Zhang, Xichen Li, Jianhua Gong, Pengtao Wang, Xiaocen Li, Xin Wang, Xin Zhang, Xu Cheng, Shuqin Yue, Taotao Zhang, Nan Parasit Vectors Research BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated. METHODS: To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction and colocalization in Giardia trophozoites were examined by using Duolink proximal ligation assay (Duolink PLA). RESULTS: From Y2H screen, the Giardia chaperone protein, Giardia DnaJ (GdDnaJ), was identified as a new binding partner for GLV RdRp. The direct interaction between GdDnaJ and GLV RdRp was verified via GST pull-down, co-immunoprecipitation and BiFC. In addition, colocalization and in vivo interaction between GdDnaJ and RdRp in Giardia trophozoites were confirmed by Duolink PLA. Further analysis revealed that KNK437, the inhibitor of GdDnaJ, can significantly reduce the replication of GLVs and the proliferation of Giardia. CONCLUSION: Taken together, our results suggested a potential role of GdDnaJ in regulating Giardia proliferation and GLV replication through interaction with GLV RdRp. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05787-0. BioMed Central 2023-05-25 /pmc/articles/PMC10210397/ /pubmed/37226181 http://dx.doi.org/10.1186/s13071-023-05787-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhang, Hongbo Zhao, Chunyan Zhang, Xichen Li, Jianhua Gong, Pengtao Wang, Xiaocen Li, Xin Wang, Xin Zhang, Xu Cheng, Shuqin Yue, Taotao Zhang, Nan A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title | A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title_full | A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title_fullStr | A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title_full_unstemmed | A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title_short | A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication |
title_sort | potential role for giardia chaperone protein gddnaj in regulating giardia proliferation and giardiavirus replication |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210397/ https://www.ncbi.nlm.nih.gov/pubmed/37226181 http://dx.doi.org/10.1186/s13071-023-05787-0 |
work_keys_str_mv | AT zhanghongbo apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhaochunyan apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangxichen apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT lijianhua apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT gongpengtao apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT wangxiaocen apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT lixin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT wangxin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangxu apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT chengshuqin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT yuetaotao apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangnan apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhanghongbo potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhaochunyan potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangxichen potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT lijianhua potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT gongpengtao potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT wangxiaocen potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT lixin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT wangxin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangxu potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT chengshuqin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT yuetaotao potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication AT zhangnan potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication |