Cargando…

A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication

BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. H...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Hongbo, Zhao, Chunyan, Zhang, Xichen, Li, Jianhua, Gong, Pengtao, Wang, Xiaocen, Li, Xin, Wang, Xin, Zhang, Xu, Cheng, Shuqin, Yue, Taotao, Zhang, Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210397/
https://www.ncbi.nlm.nih.gov/pubmed/37226181
http://dx.doi.org/10.1186/s13071-023-05787-0
_version_ 1785047059058917376
author Zhang, Hongbo
Zhao, Chunyan
Zhang, Xichen
Li, Jianhua
Gong, Pengtao
Wang, Xiaocen
Li, Xin
Wang, Xin
Zhang, Xu
Cheng, Shuqin
Yue, Taotao
Zhang, Nan
author_facet Zhang, Hongbo
Zhao, Chunyan
Zhang, Xichen
Li, Jianhua
Gong, Pengtao
Wang, Xiaocen
Li, Xin
Wang, Xin
Zhang, Xu
Cheng, Shuqin
Yue, Taotao
Zhang, Nan
author_sort Zhang, Hongbo
collection PubMed
description BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated. METHODS: To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction and colocalization in Giardia trophozoites were examined by using Duolink proximal ligation assay (Duolink PLA). RESULTS: From Y2H screen, the Giardia chaperone protein, Giardia DnaJ (GdDnaJ), was identified as a new binding partner for GLV RdRp. The direct interaction between GdDnaJ and GLV RdRp was verified via GST pull-down, co-immunoprecipitation and BiFC. In addition, colocalization and in vivo interaction between GdDnaJ and RdRp in Giardia trophozoites were confirmed by Duolink PLA. Further analysis revealed that KNK437, the inhibitor of GdDnaJ, can significantly reduce the replication of GLVs and the proliferation of Giardia. CONCLUSION: Taken together, our results suggested a potential role of GdDnaJ in regulating Giardia proliferation and GLV replication through interaction with GLV RdRp. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05787-0.
format Online
Article
Text
id pubmed-10210397
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-102103972023-05-26 A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication Zhang, Hongbo Zhao, Chunyan Zhang, Xichen Li, Jianhua Gong, Pengtao Wang, Xiaocen Li, Xin Wang, Xin Zhang, Xu Cheng, Shuqin Yue, Taotao Zhang, Nan Parasit Vectors Research BACKGROUND: Giardia duodenalis (referred to as Giardia) is a flagellated binucleate protozoan parasite, which causes one of the most common diarrheal diseases, giardiasis, worldwide. Giardia can be infected by Giardiavirus (GLV), a small endosymbiotic dsRNA virus belongs to the Totiviridae family. However, the regulation of GLV and a positive correlation between GLV and Giardia virulence is yet to be elucidated. METHODS: To identify potential regulators of GLV, we performed a yeast two-hybrid (Y2H) screen to search for interacting proteins of RdRp. GST pull-down, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assay were used to verify the direct physical interaction between GLV RdRp and its new binding partner. In addition, their in vivo interaction and colocalization in Giardia trophozoites were examined by using Duolink proximal ligation assay (Duolink PLA). RESULTS: From Y2H screen, the Giardia chaperone protein, Giardia DnaJ (GdDnaJ), was identified as a new binding partner for GLV RdRp. The direct interaction between GdDnaJ and GLV RdRp was verified via GST pull-down, co-immunoprecipitation and BiFC. In addition, colocalization and in vivo interaction between GdDnaJ and RdRp in Giardia trophozoites were confirmed by Duolink PLA. Further analysis revealed that KNK437, the inhibitor of GdDnaJ, can significantly reduce the replication of GLVs and the proliferation of Giardia. CONCLUSION: Taken together, our results suggested a potential role of GdDnaJ in regulating Giardia proliferation and GLV replication through interaction with GLV RdRp. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05787-0. BioMed Central 2023-05-25 /pmc/articles/PMC10210397/ /pubmed/37226181 http://dx.doi.org/10.1186/s13071-023-05787-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Hongbo
Zhao, Chunyan
Zhang, Xichen
Li, Jianhua
Gong, Pengtao
Wang, Xiaocen
Li, Xin
Wang, Xin
Zhang, Xu
Cheng, Shuqin
Yue, Taotao
Zhang, Nan
A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title_full A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title_fullStr A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title_full_unstemmed A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title_short A potential role for Giardia chaperone protein GdDnaJ in regulating Giardia proliferation and Giardiavirus replication
title_sort potential role for giardia chaperone protein gddnaj in regulating giardia proliferation and giardiavirus replication
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210397/
https://www.ncbi.nlm.nih.gov/pubmed/37226181
http://dx.doi.org/10.1186/s13071-023-05787-0
work_keys_str_mv AT zhanghongbo apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhaochunyan apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangxichen apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT lijianhua apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT gongpengtao apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT wangxiaocen apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT lixin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT wangxin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangxu apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT chengshuqin apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT yuetaotao apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangnan apotentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhanghongbo potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhaochunyan potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangxichen potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT lijianhua potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT gongpengtao potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT wangxiaocen potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT lixin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT wangxin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangxu potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT chengshuqin potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT yuetaotao potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication
AT zhangnan potentialroleforgiardiachaperoneproteingddnajinregulatinggiardiaproliferationandgiardiavirusreplication