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Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium
BACKGROUND: Leaves are the main medicinal organ in Epimedium herbs, and leaf flavonoid content is an important criterion of Epimedium herbs. However, the underlying genes that regulate leaf size and flavonoid content are unclear, which limits the use of breeding for Epimedium development. This study...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210407/ https://www.ncbi.nlm.nih.gov/pubmed/37231361 http://dx.doi.org/10.1186/s12870-023-04257-0 |
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author | Yu, Dongyue Huang, Ruoqi Yu, Shuxia Liang, Qiong Wang, Ying Dang, Haishan Zhang, Yanjun |
author_facet | Yu, Dongyue Huang, Ruoqi Yu, Shuxia Liang, Qiong Wang, Ying Dang, Haishan Zhang, Yanjun |
author_sort | Yu, Dongyue |
collection | PubMed |
description | BACKGROUND: Leaves are the main medicinal organ in Epimedium herbs, and leaf flavonoid content is an important criterion of Epimedium herbs. However, the underlying genes that regulate leaf size and flavonoid content are unclear, which limits the use of breeding for Epimedium development. This study focuses on QTL mapping of flavonoid and leaf-size related traits in Epimedium. RESULTS: We constructed the first high-density genetic map (HDGM) using 109 F1 hybrids of Epimedium leptorrhizum and Epimedium sagittatum over three years (2019–2021). Using 5,271 single nucleotide polymorphism (SNP) markers, an HDGM with an overall distance of 2,366.07 cM and a mean gap of 0.612 cM was generated by utilizing genotyping by sequencing (GBS) technology. Every year for three years, 46 stable quantitative trait loci (QTLs) for leaf size and flavonoid contents were discovered, including 31 stable loci for Epimedin C (EC), one stable locus for total flavone content (TFC), 12 stable loci for leaf length (LL), and two stable loci for leaf area (LA). For flavonoid content and leaf size, the phenotypic variance explained for these loci varied between 4.00 and 16.80% and 14.95 and 17.34%, respectively. CONCLUSIONS: Forty-six stable QTLs for leaf size and flavonoid content traits were repeatedly detected over three years. The HDGM and stable QTLs are laying the basis for breeding and gene investigation in Epimedium and will contribute to accelerating the identification of desirable genotypes for Epimedium breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04257-0. |
format | Online Article Text |
id | pubmed-10210407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102104072023-05-26 Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium Yu, Dongyue Huang, Ruoqi Yu, Shuxia Liang, Qiong Wang, Ying Dang, Haishan Zhang, Yanjun BMC Plant Biol Research BACKGROUND: Leaves are the main medicinal organ in Epimedium herbs, and leaf flavonoid content is an important criterion of Epimedium herbs. However, the underlying genes that regulate leaf size and flavonoid content are unclear, which limits the use of breeding for Epimedium development. This study focuses on QTL mapping of flavonoid and leaf-size related traits in Epimedium. RESULTS: We constructed the first high-density genetic map (HDGM) using 109 F1 hybrids of Epimedium leptorrhizum and Epimedium sagittatum over three years (2019–2021). Using 5,271 single nucleotide polymorphism (SNP) markers, an HDGM with an overall distance of 2,366.07 cM and a mean gap of 0.612 cM was generated by utilizing genotyping by sequencing (GBS) technology. Every year for three years, 46 stable quantitative trait loci (QTLs) for leaf size and flavonoid contents were discovered, including 31 stable loci for Epimedin C (EC), one stable locus for total flavone content (TFC), 12 stable loci for leaf length (LL), and two stable loci for leaf area (LA). For flavonoid content and leaf size, the phenotypic variance explained for these loci varied between 4.00 and 16.80% and 14.95 and 17.34%, respectively. CONCLUSIONS: Forty-six stable QTLs for leaf size and flavonoid content traits were repeatedly detected over three years. The HDGM and stable QTLs are laying the basis for breeding and gene investigation in Epimedium and will contribute to accelerating the identification of desirable genotypes for Epimedium breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04257-0. BioMed Central 2023-05-25 /pmc/articles/PMC10210407/ /pubmed/37231361 http://dx.doi.org/10.1186/s12870-023-04257-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Yu, Dongyue Huang, Ruoqi Yu, Shuxia Liang, Qiong Wang, Ying Dang, Haishan Zhang, Yanjun Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title | Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title_full | Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title_fullStr | Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title_full_unstemmed | Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title_short | Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium |
title_sort | construction of the first high-density genetic linkage map and qtl mapping of flavonoid and leaf-size related traits in epimedium |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10210407/ https://www.ncbi.nlm.nih.gov/pubmed/37231361 http://dx.doi.org/10.1186/s12870-023-04257-0 |
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