Cargando…

Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis

The collection of whole microbial communities (bacteria, archaea, fungi, and viruses) together constitutes the gut microbiome. Diet, age, stress, host genetics, and diseases cause increases or decreases in the relative abundance and diversity of bacterial species (dysbiosis). We aimed to investigate...

Descripción completa

Detalles Bibliográficos
Autores principales: Dahal, Ram Hari, Kim, Shukho, Kim, Yu Kyung, Kim, Eun Soo, Kim, Jungmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10211348/
https://www.ncbi.nlm.nih.gov/pubmed/37250025
http://dx.doi.org/10.3389/fmicb.2023.1174832
_version_ 1785047264692011008
author Dahal, Ram Hari
Kim, Shukho
Kim, Yu Kyung
Kim, Eun Soo
Kim, Jungmin
author_facet Dahal, Ram Hari
Kim, Shukho
Kim, Yu Kyung
Kim, Eun Soo
Kim, Jungmin
author_sort Dahal, Ram Hari
collection PubMed
description The collection of whole microbial communities (bacteria, archaea, fungi, and viruses) together constitutes the gut microbiome. Diet, age, stress, host genetics, and diseases cause increases or decreases in the relative abundance and diversity of bacterial species (dysbiosis). We aimed to investigate the gut microbial composition at different taxonomic levels of healthy controls (HCs) with active Crohn’s disease (CD), ulcerative colitis (UC), and ischemic colitis (IC) using culture- and non-culture-based approaches and identify biomarkers to discriminate CD, UC, or IC. We determined the specific changes in the gut microbial profile using culture-independent (16S rRNA gene amplicon sequencing) as well as culture-based (culturomic) approaches. Biomarkers were validated using quantitative Real-Time PCR (qPCR). In both methods, bacterial diversity and species richness decreased in disease-associated conditions compared with that in HCs. Highly reduced abundance of Faecalibacterium prausnitzii and Prevotella sp. and an increased abundance of potentially pathogenic bacteria such as Enterococcus faecium, Enterococcus faecalis, and Escherichia coli in all CD, UC, or IC conditions were observed. We noted a high abundance of Latilactobacillus sakei in CD patients; Ligilactobacillus ruminis in UC patients; and Enterococcus faecium, Escherichia coli, and Enterococcus faecalis in IC patients. Highly reduced abundance of Faecalibacterium prausnitzii in all cases, and increased abundance of Latilactobacillus sakei and Enterococcus faecium in CD, Ligilactobacillus ruminis and Enterococcus faecium in UC, and Enterococcus faecium, Escherichia coli, and Enterococcus faecalis in IC could be biomarkers for CD, UC, and IC, respectively. These biomarkers may help in IBD (CD or UC) and IC diagnosis.
format Online
Article
Text
id pubmed-10211348
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102113482023-05-26 Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis Dahal, Ram Hari Kim, Shukho Kim, Yu Kyung Kim, Eun Soo Kim, Jungmin Front Microbiol Microbiology The collection of whole microbial communities (bacteria, archaea, fungi, and viruses) together constitutes the gut microbiome. Diet, age, stress, host genetics, and diseases cause increases or decreases in the relative abundance and diversity of bacterial species (dysbiosis). We aimed to investigate the gut microbial composition at different taxonomic levels of healthy controls (HCs) with active Crohn’s disease (CD), ulcerative colitis (UC), and ischemic colitis (IC) using culture- and non-culture-based approaches and identify biomarkers to discriminate CD, UC, or IC. We determined the specific changes in the gut microbial profile using culture-independent (16S rRNA gene amplicon sequencing) as well as culture-based (culturomic) approaches. Biomarkers were validated using quantitative Real-Time PCR (qPCR). In both methods, bacterial diversity and species richness decreased in disease-associated conditions compared with that in HCs. Highly reduced abundance of Faecalibacterium prausnitzii and Prevotella sp. and an increased abundance of potentially pathogenic bacteria such as Enterococcus faecium, Enterococcus faecalis, and Escherichia coli in all CD, UC, or IC conditions were observed. We noted a high abundance of Latilactobacillus sakei in CD patients; Ligilactobacillus ruminis in UC patients; and Enterococcus faecium, Escherichia coli, and Enterococcus faecalis in IC patients. Highly reduced abundance of Faecalibacterium prausnitzii in all cases, and increased abundance of Latilactobacillus sakei and Enterococcus faecium in CD, Ligilactobacillus ruminis and Enterococcus faecium in UC, and Enterococcus faecium, Escherichia coli, and Enterococcus faecalis in IC could be biomarkers for CD, UC, and IC, respectively. These biomarkers may help in IBD (CD or UC) and IC diagnosis. Frontiers Media S.A. 2023-05-11 /pmc/articles/PMC10211348/ /pubmed/37250025 http://dx.doi.org/10.3389/fmicb.2023.1174832 Text en Copyright © 2023 Dahal, Kim, Kim, Kim and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dahal, Ram Hari
Kim, Shukho
Kim, Yu Kyung
Kim, Eun Soo
Kim, Jungmin
Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title_full Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title_fullStr Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title_full_unstemmed Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title_short Insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
title_sort insight into gut dysbiosis of patients with inflammatory bowel disease and ischemic colitis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10211348/
https://www.ncbi.nlm.nih.gov/pubmed/37250025
http://dx.doi.org/10.3389/fmicb.2023.1174832
work_keys_str_mv AT dahalramhari insightintogutdysbiosisofpatientswithinflammatoryboweldiseaseandischemiccolitis
AT kimshukho insightintogutdysbiosisofpatientswithinflammatoryboweldiseaseandischemiccolitis
AT kimyukyung insightintogutdysbiosisofpatientswithinflammatoryboweldiseaseandischemiccolitis
AT kimeunsoo insightintogutdysbiosisofpatientswithinflammatoryboweldiseaseandischemiccolitis
AT kimjungmin insightintogutdysbiosisofpatientswithinflammatoryboweldiseaseandischemiccolitis