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A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis
Non-alcoholic fatty liver disease (NAFLD) has a high prevalence worldwide, with a significant proportion of patients progressing into non-alcoholic steatohepatitis (NASH) and further into cirrhosis and hepatocellular carcinoma (HCC). Most of the current animal models of NASH have limitations, such a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212180/ https://www.ncbi.nlm.nih.gov/pubmed/37228085 http://dx.doi.org/10.1371/journal.pone.0286257 |
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author | Li, Xuecheng Lu, Yi Liang, Xiaoshuang Zhou, Xiaofei Li, Dirui Zhang, Zan Niu, Yunchao Liu, Shuaishuai Ye, Ling Zhang, Rufeng |
author_facet | Li, Xuecheng Lu, Yi Liang, Xiaoshuang Zhou, Xiaofei Li, Dirui Zhang, Zan Niu, Yunchao Liu, Shuaishuai Ye, Ling Zhang, Rufeng |
author_sort | Li, Xuecheng |
collection | PubMed |
description | Non-alcoholic fatty liver disease (NAFLD) has a high prevalence worldwide, with a significant proportion of patients progressing into non-alcoholic steatohepatitis (NASH) and further into cirrhosis and hepatocellular carcinoma (HCC). Most of the current animal models of NASH have limitations, such as incompatibility with human pathogenesis characteristics or long induction periods, which severely limit the development of new drugs and preclinical studies for NASH. We investigated the progression of NASH and fibrosis, as well as metabolic indicators, at different time points in aged mice induced by the Gubra Amylin NASH (GAN) diet, a high-fat, high-sugar, high-cholesterol diet, and attempted to establish a rapid and useful mouse model of NASH. Young and aged C57BL/6 mice were induced on a normal chow or GAN diet for 12 and 21 weeks, respectively. After 12 weeks of induction, aged mice developed NASH, including hepatic steatosis, lobular inflammation and hepatic ballooning, and the phenotype was more severe compared with young mice. After 21 weeks of induction, aged mice developed hepatic fibrosis, which greatly shortened the induction time compared with young mice. Furthermore, analysis of immune cell infiltration in the liver by flow cytometry elucidated the changes of multiple immune cells during the pathogenesis of NASH. These findings suggest that aged mice may develop NASH and fibrosis more rapidly under GAN diet induction, which may significantly shorten the period for preclinical studies of NASH. |
format | Online Article Text |
id | pubmed-10212180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102121802023-05-26 A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis Li, Xuecheng Lu, Yi Liang, Xiaoshuang Zhou, Xiaofei Li, Dirui Zhang, Zan Niu, Yunchao Liu, Shuaishuai Ye, Ling Zhang, Rufeng PLoS One Research Article Non-alcoholic fatty liver disease (NAFLD) has a high prevalence worldwide, with a significant proportion of patients progressing into non-alcoholic steatohepatitis (NASH) and further into cirrhosis and hepatocellular carcinoma (HCC). Most of the current animal models of NASH have limitations, such as incompatibility with human pathogenesis characteristics or long induction periods, which severely limit the development of new drugs and preclinical studies for NASH. We investigated the progression of NASH and fibrosis, as well as metabolic indicators, at different time points in aged mice induced by the Gubra Amylin NASH (GAN) diet, a high-fat, high-sugar, high-cholesterol diet, and attempted to establish a rapid and useful mouse model of NASH. Young and aged C57BL/6 mice were induced on a normal chow or GAN diet for 12 and 21 weeks, respectively. After 12 weeks of induction, aged mice developed NASH, including hepatic steatosis, lobular inflammation and hepatic ballooning, and the phenotype was more severe compared with young mice. After 21 weeks of induction, aged mice developed hepatic fibrosis, which greatly shortened the induction time compared with young mice. Furthermore, analysis of immune cell infiltration in the liver by flow cytometry elucidated the changes of multiple immune cells during the pathogenesis of NASH. These findings suggest that aged mice may develop NASH and fibrosis more rapidly under GAN diet induction, which may significantly shorten the period for preclinical studies of NASH. Public Library of Science 2023-05-25 /pmc/articles/PMC10212180/ /pubmed/37228085 http://dx.doi.org/10.1371/journal.pone.0286257 Text en © 2023 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Xuecheng Lu, Yi Liang, Xiaoshuang Zhou, Xiaofei Li, Dirui Zhang, Zan Niu, Yunchao Liu, Shuaishuai Ye, Ling Zhang, Rufeng A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title | A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title_full | A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title_fullStr | A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title_full_unstemmed | A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title_short | A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis |
title_sort | new nash model in aged mice with rapid progression of steatohepatitis and fibrosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212180/ https://www.ncbi.nlm.nih.gov/pubmed/37228085 http://dx.doi.org/10.1371/journal.pone.0286257 |
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