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Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea
Sensorineural hearing loss a result from hair cell damage, which is irreversible in mammals owing to the lack of hair cell regeneration, but recent researches have shown that Lgr5 (+) supporting cells are progenitors capable of regenerating hair cells. RPS14 (ribosomal protein S14) is a 40S ribosoma...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212696/ https://www.ncbi.nlm.nih.gov/pubmed/36977657 http://dx.doi.org/10.1111/cpr.13458 |
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author | Xu, Changling Qi, Jieyu Hu, Xiaojie Zhang, Liyan Sun, Qiuhan Li, Nianci Chen, Xin Guo, Fangfang Wu, Peina Shi, Yi Chai, Renjie |
author_facet | Xu, Changling Qi, Jieyu Hu, Xiaojie Zhang, Liyan Sun, Qiuhan Li, Nianci Chen, Xin Guo, Fangfang Wu, Peina Shi, Yi Chai, Renjie |
author_sort | Xu, Changling |
collection | PubMed |
description | Sensorineural hearing loss a result from hair cell damage, which is irreversible in mammals owing to the lack of hair cell regeneration, but recent researches have shown that Lgr5 (+) supporting cells are progenitors capable of regenerating hair cells. RPS14 (ribosomal protein S14) is a 40S ribosomal subunit component and is associated with erythrocyte differentiation, and in this study, we used a novel adeno‐associated virus‐inner ear system to upregulate Rps14 expression in cultured hair cell progenitors and observed an enhancement on their ability to proliferate and differentiate into hair cells. Similarly, Rps14 overexpression in the mice cochlea could promote supporting cells proliferation by activating the Wnt signalling pathway. In addition, over‐expressing Rps14 induced hair cells regeneration in the organ of Corti, and lineage tracing showed that the new hair cells had transformed from Lgr5(+) progenitors. In conclusion, our analysis reveals the potential role of Rps14 in driving hair cell regeneration in mammalian. |
format | Online Article Text |
id | pubmed-10212696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102126962023-05-27 Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea Xu, Changling Qi, Jieyu Hu, Xiaojie Zhang, Liyan Sun, Qiuhan Li, Nianci Chen, Xin Guo, Fangfang Wu, Peina Shi, Yi Chai, Renjie Cell Prolif Original Articles Sensorineural hearing loss a result from hair cell damage, which is irreversible in mammals owing to the lack of hair cell regeneration, but recent researches have shown that Lgr5 (+) supporting cells are progenitors capable of regenerating hair cells. RPS14 (ribosomal protein S14) is a 40S ribosomal subunit component and is associated with erythrocyte differentiation, and in this study, we used a novel adeno‐associated virus‐inner ear system to upregulate Rps14 expression in cultured hair cell progenitors and observed an enhancement on their ability to proliferate and differentiate into hair cells. Similarly, Rps14 overexpression in the mice cochlea could promote supporting cells proliferation by activating the Wnt signalling pathway. In addition, over‐expressing Rps14 induced hair cells regeneration in the organ of Corti, and lineage tracing showed that the new hair cells had transformed from Lgr5(+) progenitors. In conclusion, our analysis reveals the potential role of Rps14 in driving hair cell regeneration in mammalian. John Wiley and Sons Inc. 2023-03-28 /pmc/articles/PMC10212696/ /pubmed/36977657 http://dx.doi.org/10.1111/cpr.13458 Text en © 2023 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Xu, Changling Qi, Jieyu Hu, Xiaojie Zhang, Liyan Sun, Qiuhan Li, Nianci Chen, Xin Guo, Fangfang Wu, Peina Shi, Yi Chai, Renjie Rps14 upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title |
Rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title_full |
Rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title_fullStr |
Rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title_full_unstemmed |
Rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title_short |
Rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
title_sort | rps14
upregulation promotes inner ear progenitor proliferation and hair cell regeneration in the neonatal mouse cochlea |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212696/ https://www.ncbi.nlm.nih.gov/pubmed/36977657 http://dx.doi.org/10.1111/cpr.13458 |
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