Viewing life without labels under optical microscopes

Optical microscopes today have pushed the limits of speed, quality, and observable space in biological specimens revolutionizing how we view life today. Further, specific labeling of samples for imaging has provided insight into how life functions. This enabled label-based microscopy to percolate an...

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Detalles Bibliográficos
Autores principales: Ghosh, Biswajoy, Agarwal, Krishna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212946/
https://www.ncbi.nlm.nih.gov/pubmed/37231084
http://dx.doi.org/10.1038/s42003-023-04934-8
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author Ghosh, Biswajoy
Agarwal, Krishna
author_facet Ghosh, Biswajoy
Agarwal, Krishna
author_sort Ghosh, Biswajoy
collection PubMed
description Optical microscopes today have pushed the limits of speed, quality, and observable space in biological specimens revolutionizing how we view life today. Further, specific labeling of samples for imaging has provided insight into how life functions. This enabled label-based microscopy to percolate and integrate into mainstream life science research. However, the use of labelfree microscopy has been mostly limited, resulting in testing for bio-application but not bio-integration. To enable bio-integration, such microscopes need to be evaluated for their timeliness to answer biological questions uniquely and establish a long-term growth prospect. The article presents key label-free optical microscopes and discusses their integrative potential in life science research for the unperturbed analysis of biological samples.
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spelling pubmed-102129462023-05-27 Viewing life without labels under optical microscopes Ghosh, Biswajoy Agarwal, Krishna Commun Biol Perspective Optical microscopes today have pushed the limits of speed, quality, and observable space in biological specimens revolutionizing how we view life today. Further, specific labeling of samples for imaging has provided insight into how life functions. This enabled label-based microscopy to percolate and integrate into mainstream life science research. However, the use of labelfree microscopy has been mostly limited, resulting in testing for bio-application but not bio-integration. To enable bio-integration, such microscopes need to be evaluated for their timeliness to answer biological questions uniquely and establish a long-term growth prospect. The article presents key label-free optical microscopes and discusses their integrative potential in life science research for the unperturbed analysis of biological samples. Nature Publishing Group UK 2023-05-25 /pmc/articles/PMC10212946/ /pubmed/37231084 http://dx.doi.org/10.1038/s42003-023-04934-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Perspective
Ghosh, Biswajoy
Agarwal, Krishna
Viewing life without labels under optical microscopes
title Viewing life without labels under optical microscopes
title_full Viewing life without labels under optical microscopes
title_fullStr Viewing life without labels under optical microscopes
title_full_unstemmed Viewing life without labels under optical microscopes
title_short Viewing life without labels under optical microscopes
title_sort viewing life without labels under optical microscopes
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10212946/
https://www.ncbi.nlm.nih.gov/pubmed/37231084
http://dx.doi.org/10.1038/s42003-023-04934-8
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