Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. new bacteria isolated from human stool microbiota

Two new bacterial strains, Marseille-P2698(T) (CSUR P2698 = DSM 103,121) and Marseille-P2260(T) (CSUR P2260 = DSM 101,844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach to fully describe these two new bacterial strains. The Marseille-P2698(T) strain was a Gram-negative, motile, non-spore-forming, rod-shaped bacterium. The Marseille-P2260(T) strain was a Gram-positive, motile, spore-forming rod-shaped bacterium. Major fatty acids found in Marseille-P2698(T) were C(15:0 iso) (63%), C(15:0 anteiso) (11%), and C(17:0 3-OH iso) (8%). Those found in Marseille-P2260(T) strain were C(16:00) (39%), C(18:1n9) (16%) and C(18:1n7) (14%). Strains Marseille-P2698(T) and Marseille-P2260(T) had 16S rRNA gene sequence similarities of 91.50% with Odoribacter laneus(T), and of 90.98% and 95.07% with Odoribacter splanchnicus(T) and Eubacterium sulci(T), respectively. The exhibited digital DNA-DNA Hybridization values lower than 20.7%, and Orthologous Average Nucleotide Identity values lower than 73% compared to their closest related bacterial species O. splanchnicus(T) and E. sulci(T) respectively. Phenotypic, biochemical, phylogenetic, and genomic results obtained by comparative analyses provided sufficient evidence that both of the two studied strains Marseille-P2698(T) and Marseille-P2260(T) are two new bacterial species and new bacterial genera for which the names Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. were proposed, respectively.