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Direct Adeno-associated Viruses Injection of Murine Adipose Tissue
The adipose tissue is a central metabolic organ that regulates whole-body energy homeostasis. The abnormal expansion of adipose tissue leads to the progression of obesity. The adipose tissue microenvironment is affected by pathological hypertrophy of adipocytes, highly correlated with systemic metab...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213077/ https://www.ncbi.nlm.nih.gov/pubmed/37251093 http://dx.doi.org/10.21769/BioProtoc.4674 |
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author | Wu, Shao-Chin Lin, Chi-Hung |
author_facet | Wu, Shao-Chin Lin, Chi-Hung |
author_sort | Wu, Shao-Chin |
collection | PubMed |
description | The adipose tissue is a central metabolic organ that regulates whole-body energy homeostasis. The abnormal expansion of adipose tissue leads to the progression of obesity. The adipose tissue microenvironment is affected by pathological hypertrophy of adipocytes, highly correlated with systemic metabolic disorders. In vivo genetic modification is a great tool for understanding the role of genes involved in such processes. However, obtaining new conventional engineered mice is time consuming and costly. Here, we provide a simple and speedy method to efficiently transduce genes into adipose tissue by injecting the adeno-associated virus vector serotypes 8 (AAV8) into the fat pads of adult mice. |
format | Online Article Text |
id | pubmed-10213077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Bio-Protocol |
record_format | MEDLINE/PubMed |
spelling | pubmed-102130772023-05-27 Direct Adeno-associated Viruses Injection of Murine Adipose Tissue Wu, Shao-Chin Lin, Chi-Hung Bio Protoc Methods Article The adipose tissue is a central metabolic organ that regulates whole-body energy homeostasis. The abnormal expansion of adipose tissue leads to the progression of obesity. The adipose tissue microenvironment is affected by pathological hypertrophy of adipocytes, highly correlated with systemic metabolic disorders. In vivo genetic modification is a great tool for understanding the role of genes involved in such processes. However, obtaining new conventional engineered mice is time consuming and costly. Here, we provide a simple and speedy method to efficiently transduce genes into adipose tissue by injecting the adeno-associated virus vector serotypes 8 (AAV8) into the fat pads of adult mice. Bio-Protocol 2023-05-20 /pmc/articles/PMC10213077/ /pubmed/37251093 http://dx.doi.org/10.21769/BioProtoc.4674 Text en Copyright © 2023 The Authors; exclusive licensee Bio-protocol LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/). |
spellingShingle | Methods Article Wu, Shao-Chin Lin, Chi-Hung Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title | Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title_full | Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title_fullStr | Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title_full_unstemmed | Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title_short | Direct Adeno-associated Viruses Injection of Murine Adipose Tissue |
title_sort | direct adeno-associated viruses injection of murine adipose tissue |
topic | Methods Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213077/ https://www.ncbi.nlm.nih.gov/pubmed/37251093 http://dx.doi.org/10.21769/BioProtoc.4674 |
work_keys_str_mv | AT wushaochin directadenoassociatedvirusesinjectionofmurineadiposetissue AT linchihung directadenoassociatedvirusesinjectionofmurineadiposetissue |