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Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis

The secondary palate forms from two lateral primordia called the palatal shelves which form a contact in the midline, become adherent at the fusing interface (medial edge epithelia, MEE) and subsequently fuse. The gene encoding transforming growth factor-ß3 (Tgfb3) is strongly and specifically expre...

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Autores principales: Saroya, Ghazi-Abdullah, Siismets, Erica, Hu, Max, Panaretos, Christopher, Rice, Adam, Reynolds, Kurt, Zhou, Chengji J., Kaartinen, Vesa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213314/
https://www.ncbi.nlm.nih.gov/pubmed/37250135
http://dx.doi.org/10.3389/fphys.2023.704406
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author Saroya, Ghazi-Abdullah
Siismets, Erica
Hu, Max
Panaretos, Christopher
Rice, Adam
Reynolds, Kurt
Zhou, Chengji J.
Kaartinen, Vesa
author_facet Saroya, Ghazi-Abdullah
Siismets, Erica
Hu, Max
Panaretos, Christopher
Rice, Adam
Reynolds, Kurt
Zhou, Chengji J.
Kaartinen, Vesa
author_sort Saroya, Ghazi-Abdullah
collection PubMed
description The secondary palate forms from two lateral primordia called the palatal shelves which form a contact in the midline, become adherent at the fusing interface (medial edge epithelia, MEE) and subsequently fuse. The gene encoding transforming growth factor-ß3 (Tgfb3) is strongly and specifically expressed in MEE cells. Our previous study suggested that Tgfb3 expression is controlled via upstream cis-regulatory elements in and around the neighboring Ift43 gene. Another study suggested that the canonical Wnt signaling via ß-Catenin is responsible for the MEE-specific Tgfb3 gene expression, since deletion of the Ctnnb1 gene by a commonly used Keratin 14-Cre (K14Cre) mouse line almost completely abolished Tgfb3 expression in the MEE resulting in cleft palate. Here, we wanted to analyze whether Tcf/Lef consensus binding sites located in the previously identified regions of the Ift43 gene are responsible for the spatiotemporal control of Tgfb3 expression during palatogenesis. We show that contrary to the previous report, deletion of the Ctnnb1 gene in basal MEE cells by the K14Cre driver (the same K14Cre mouse line was used as in the previous study referenced above) does not affect the MEE-specific Tgfb3 expression or TGFß3-dependent palatal epithelial fusion. All mutant embryos showed a lack of palatal rugae accompanied by other craniofacial defects, e.g., a narrow snout and a small upper lip, while only a small subset (<5%) of Ctnnb1 mutants displayed a cleft palate. Moreover, the K14Cre:Ctnnb1 embryos showed reduced levels and altered patterns of Shh expression. Our present data imply that epithelial ß-catenin may not be required for MEE-specific Tgfb3 expression or palatal epithelial fusion.
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spelling pubmed-102133142023-05-27 Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis Saroya, Ghazi-Abdullah Siismets, Erica Hu, Max Panaretos, Christopher Rice, Adam Reynolds, Kurt Zhou, Chengji J. Kaartinen, Vesa Front Physiol Physiology The secondary palate forms from two lateral primordia called the palatal shelves which form a contact in the midline, become adherent at the fusing interface (medial edge epithelia, MEE) and subsequently fuse. The gene encoding transforming growth factor-ß3 (Tgfb3) is strongly and specifically expressed in MEE cells. Our previous study suggested that Tgfb3 expression is controlled via upstream cis-regulatory elements in and around the neighboring Ift43 gene. Another study suggested that the canonical Wnt signaling via ß-Catenin is responsible for the MEE-specific Tgfb3 gene expression, since deletion of the Ctnnb1 gene by a commonly used Keratin 14-Cre (K14Cre) mouse line almost completely abolished Tgfb3 expression in the MEE resulting in cleft palate. Here, we wanted to analyze whether Tcf/Lef consensus binding sites located in the previously identified regions of the Ift43 gene are responsible for the spatiotemporal control of Tgfb3 expression during palatogenesis. We show that contrary to the previous report, deletion of the Ctnnb1 gene in basal MEE cells by the K14Cre driver (the same K14Cre mouse line was used as in the previous study referenced above) does not affect the MEE-specific Tgfb3 expression or TGFß3-dependent palatal epithelial fusion. All mutant embryos showed a lack of palatal rugae accompanied by other craniofacial defects, e.g., a narrow snout and a small upper lip, while only a small subset (<5%) of Ctnnb1 mutants displayed a cleft palate. Moreover, the K14Cre:Ctnnb1 embryos showed reduced levels and altered patterns of Shh expression. Our present data imply that epithelial ß-catenin may not be required for MEE-specific Tgfb3 expression or palatal epithelial fusion. Frontiers Media S.A. 2023-05-12 /pmc/articles/PMC10213314/ /pubmed/37250135 http://dx.doi.org/10.3389/fphys.2023.704406 Text en Copyright © 2023 Saroya, Siismets, Hu, Panaretos, Rice, Reynolds, Zhou and Kaartinen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Saroya, Ghazi-Abdullah
Siismets, Erica
Hu, Max
Panaretos, Christopher
Rice, Adam
Reynolds, Kurt
Zhou, Chengji J.
Kaartinen, Vesa
Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title_full Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title_fullStr Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title_full_unstemmed Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title_short Canonical Wnt signaling is not required for Tgfb3 expression in the basal medial edge epithelium during palatogenesis
title_sort canonical wnt signaling is not required for tgfb3 expression in the basal medial edge epithelium during palatogenesis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213314/
https://www.ncbi.nlm.nih.gov/pubmed/37250135
http://dx.doi.org/10.3389/fphys.2023.704406
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